Article
- The EMBO Journal (1998) 17, 4283 - 4290
- doi:10.1093/emboj/17.15.4283
A mammalian two pore domain mechano-gated S-like K+ channel
Amanda J. Patel1,2, Eric Honoré1,2, François Maingret1, Florian Lesage1, Michel Fink1, Fabrice Duprat1 and Michel Lazdunski1
- Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, 660 route des Lucioles, Sophia Antipolis, 06560 Valbonne, France
- A.J.Patel and E.Honoré contributed equally to this work
Correspondence to:
Michel Lazdunski, E-mail: ipmc@ipmc.cnrs.fr
Received 22 April 1998; Accepted 2 June 1998; Revised 26 May 1998
Abstract
Aplysia S-type K+ channels of sensory neurons play a dominant role in presynaptic facilitation and behavioural sensitization. They are closed by serotonin via cAMP-dependent phosphorylation, whereas they are opened by arachidonic acid, volatile general anaesthetics and mechanical stimulation. We have identified a cloned mammalian two P domain K+ channel sharing the properties of the S channel. In addition, the recombinant channel is opened by lipid bilayer amphipathic crenators, while it is closed by cup-formers. The cytoplasmic C-terminus contains a charged region critical for chemical and mechanical activation, as well as a phosphorylation site required for cAMP inhibition.
Keywords:
- arachidonic acid,
- K+ channel,
- serotonin,
- stretch,
- volatile general anaesthetics
