Figure 3
Stat1 combines signals derived from IFN-
and LPS receptors during macrophage activation
Pavel Kovarik, Dagmar Stoiber, Michael Novy and Thomas Decker
- The EMBO Journal (1998) 17, 3660 - 3668
- doi:10.1093/emboj/17.13.3660
The effect of bacteria, LPS or CSF-1 on Stat1 S727 phosphorylation in mouse macrophages. (A)Bac1.2F5 macrophages were either infected with S.typhimurium or treated with LPS for 20 min. Stimulation with human recombinant CSF-1 was for 10 min. Determination of Stat1 S727 phosphorylation was with anti-pS727 as described for Figure 1. (B)Extracts from Bac1.2F5 cells after LPS treatment or Salmonella infection were assayed for tyrosine phosphorylation of Stats in the EMSA with a 
-casein GAS sequence as a labeled probe.
