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The EMBO Journal (1998) 17, 3640–3650, doi:10.1093/emboj/17.13.3640 Figure 1 Overlapping functions of components of a bacterial Sec-independent protein export pathway Frank Sargent, Erik G. Bogsch, Nicola R. Stanley, Margaret Wexler, Colin Robinson, Ben C. Berks and Tracy Palmer

Figure 1 The tat locus and in vivo synthesis of TatA–D. (A) A partial restriction map of the tat locus, showing the positions of the tat genes after DNA sequence correction. Plasmid pFAT65 carries 2.5 kb covering tatABCD cloned distal to the phage T7 10 promoter of plasmid pT7.5 as described in the text. The regions of deleted DNA in plasmids pFAT66–pFAT68 are indicated in the figure. Restriction site abbreviations are: E, EcoRI; H, HindIII; N, NruI; R, EcoRV; X, XbaI. (B) In vivo synthesis of the tat gene products from plasmids pFAT65–pFAT68. Autoradiograph of a 12.5% gel after SDS–PAGE. Total proteins were prepared from K38/pGP1-2 containing the following plasmids: lane 1, pT7.5; lane 2, pFAT65; lane 3, pFAT66; lane 4, pFAT67; lane 5, pFAT68.

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