Figure 2
Embryonic lethality and abnormal cardiac myocytes in mice lacking ryanodine receptor type 2
Hiroshi Takeshima, Shinji Komazaki, Kenzo Hirose, Miyuki Nishi, Tetsuo Noda and Masamitsu Iino
- The EMBO Journal (1998) 17, 3309 - 3316
- doi:10.1093/emboj/17.12.3309
Detection of mutant RyR-2 genes. (A) Southern blot analysis of DNAs from ES clones containing mutant RyR-2 genes. DNAs from ES cells were digested with EcoRI and analysed. The hybridization probe (probe 1) used and the expected sizes of the positive restriction fragments are shown in Figure 1. ES clones #154 and #A7 contain the crrm1 and crrm2 mutant genes, respectively. (B) Detection of the crrm2 mutant gene by PCR using genomic DNAs from mouse embryos. Primers used for amplification and the expected sizes of the products are shown in Figure 1. The size markers are indicated in kilobase pairs.
