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The EMBO Journal (1998) 17, 2830–2837, doi:10.1093/emboj/17.10.2830 Figure 6 Sphingosine kinase-mediated Ca2+ signalling by G-protein-coupled receptors Dagmar Meyer zu Heringdorf, Holger Lass, Regina Alemany, Kai T. Laser, Eva Neumann, Chunyi Zhang, Martina Schmidt, Ursula Rauen, Karl H. Jakobs and Chris J. van Koppen

Figure 6 Fura-2 imaging of single HEK-293 cells injected with SPP and IP3. [Ca2+]i was measured in adherent m2 mAChR-expressing HEK-293 cells pretreated with PTX (75 ng/ml, 16 h). In the panels, several individual fluorescence traces representative of between 8 and 40 recordings are shown. Arrows indicate time points of injection. Injection buffer solution contained (A) vehicle (control), (B) 1 mM SPP, (C) 100 M SPP, (D) 10 M SPP, (E) 1 mM SPP after pretreatment for 30–120 s with 5 mM EGTA, or (F) 1 mM IP3.

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