Sphingosine kinase-mediated Ca2+ signalling by G-protein-coupled receptors

The EMBO Journal (1998) 17, 2830–2837, doi:10.1093/emboj/17.10.2830

Figure 5

Sphingosine kinase-mediated Ca²⁺ signalling by G-protein-coupled receptors

Dagmar Meyer zu Heringdorf, Holger Lass, Regina Alemany, Kai T. Laser, Eva Neumann, Chunyi Zhang, Martina Schmidt, Ursula Rauen, Karl H. Jakobs and Chris J. van Koppen

Figure 5
Stimulation of [³H]SPP formation by m2 and m3 mAChRs. Formation of [³H]SPP from [³H]sphingosine was determined in m2 and m3 mAChR-expressing HEK-293 cells for the indicated periods in the absence and presence of 100

M (m2 mAChR) or 0.3

M (m3 mAChR) carbachol. Values are expressed as percentage increase relative to the radioactivity in unstimulated cells and are mean plusminus

SEM of three or more independent experiments, each carried out at least in triplicate. Incubation with carbachol did not alter the uptake of [³H]sphingosine. ^*Significantly different from unstimulated cells (p <0.05, two-tailed paired t-test).


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