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The EMBO Journal (1997) 16, 1809–1819, doi:10.1093/emboj/16.8.1809 Figure 4 Essential role for -tubulin in the acentriolar female meiotic spindle of Drosophila Gaia Tavosanis, Salud Llamazares, George Goulielmos and Cayetano Gonzalez

Figure 4 Developmental expression of the TUB37C polypeptide. Western blot hybridized with the Rb1011 antibody. The lines contain protein extracts from different tissues as indicated. Egg chamber stages are as defined by King (1970). Tissues were obtained from individuals which were wild-type (wt/wt), fs(2)TW11 homozygous (mut/mut), fs(2)TW11 hemizygous over Df(2L)VA23 (Df/mut) and fs(2)TW11 over the same deficiency plus the P[TUB37C+ w+] transgene that carries one copy of the TUB37C gene (P[+]/Df/mut). Balanced loading was controlled by scanning and quantification following Ponceau staining of the membrane before incubation with the antibody. The region of the scanned membrane containing the major bands present in ovarioles and early embryos is shown at the bottom of the figure.

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