|
 |
|
|
| The EMBO Journal
(1997) 16, 685–694, doi:10.1093/emboj/16.4.685 |
|
| A mouse cytomegalovirus glycoprotein, gp34, forms a complex with folded class I MHC molecules in the ER which is not retained but is transported to the cell surface |
|
|
| Maurits F. Kleijnen2, Johannes B. Huppa1, Pero Lucin3, Siddhartha Mukherjee1, Helen Farrell4, Ann E. Campbell5, Ulrich H. Koszinowski6, Ann B. Hill7 and Hidde L. Ploegh2 |
|
|
1 Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street, Cambridge, MA 02139, USA 2 Present address: Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA 3 Department of Physiology and Immunology, University of Rijeka, 51000 Rijeka, Croatia 4 Department of Microbiology, University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands, Australia 5 Department of Microbiology and Immunology, Eastern Virginia Medical School, Norfolk, VA 23507, USA 6 Max von Pettenkofer Institut der Ludwig-Maximilians-Universität, 80336 München, Germany 7 Present address: Department of Molecular Microbiology and Immunology, OHSU, L220, 3181 SW Sam Jackson Park Road, Portland, OR 97201-3098, USA Received 30 August 1996; Revised 4 November 1996. |
|
|
|
| Abstract |
|
| Murine cytomegalovirus (MCMV) interferes with antigen presentation by means of retaining major histocompatibility complex (MHC) class I molecules in the endoplasmic reticulum (ER). Here we identify and characterize an MCMV-encoded glycoprotein, gp34, which tightly associates with properly conformed MHC class I molecules in the ER. Gp34 is synthesized in large quantities during MCMV infection and it leaves the ER only in association with MHC class I complexes. Many but not all class I molecules are retained in the ER during the early phase of MCMV infection, and we observe an inverse correlation between amounts of gp34 synthesized during the course of infection and class I retention. An MCMV deletion mutant lacking several genes, including the gene encoding gp34, shows increased class I retention. Thus, MCMV gp34 may counteract class I retention, perhaps to decrease susceptibility of infected cells to recognition by natural killer cells. |
|
| Keywords: antigen presentation, ER retention, gp34, MHC class I, murine cytomegalovirus |
|
|
| |
| |
| |
 | Email link to a friend |
| |
 | Download PDF |
| |
 | Full text |
| |
| |
| |
 | Next article |
| |
 | Previous article |
| |
 | Table of contents |
| |
| |
 | Rights and permissions |
| |
 | Reprints |
| |
| |
| |
 Register for table of contents by email | |
| |
| |
| |
