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The EMBO Journal (1997) 16, 7422–7431, doi:10.1093/emboj/16.24.7422 Figure 6 Position-dependent and promoter-specific regulation of gene expression in Trypanosoma brucei David Horn and George A.M. Cross

Figure 6 Neo expression in the vsg221 ES in individual cells. Rabbit anti-Neo primary antibody and rhodamine-conjugated goat anti-rabbit secondary antibody were used to detect Neo (red). DAPI (Sigma) was used to stain nuclear and mitochondrial DNA (blue). In bloodstream forms Neo expression was driven by the endogenous active ES promoter (A) or an ES promoter integrated at a repressed ES (B). In procyclic forms Neo expression was driven by an ES promoter within an ES (C) or an rRNA promoter within an ES (D).

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