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Figure 5
Transcription repression at the non-telomeric vsg118 locus. (A) Physical map of the ble–promoter–neo cassette and the integration site (vertical arrow). (B) A Northern blot was hybridized with the probes indicated to the left of each panel. BF, bloodstream forms; PF, procyclic forms; R, cassette with rRNA promoter; E, cassette with ES promoter. Cells with a cassette integrated in an active ES are shown as a positive control (active ES). (C) neo signals were quantified by phosphorimager analysis. The numbers above each bar represent the number of independent clones in each data set. Standard deviation was negligible in these samples. Symbols are as in (B).
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