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Figure 1
Physical maps of the vsg221 expression site and T.brucei promoters. (A) Salient non-coding motifs in the vsg221 ES. The arrow represents the vsg ES promoter. Repeats that occur in tandem arrays are labeled below the map. Other sequences of interest are labeled above the map; 8/16, octamer/hexadecamer conserved sequences; STR, sub-telomeric repeat. (B) The promoters used in this study are shown. The arrows indicate the transcription initiation points, which are designated as position +1. Black boxes upstream of the initiation point indicate regions encompassing elements that are critical for promoter activity in transient transfection assays.
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