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Figure 3

Regulation of the pp72syk protein tyrosine kinase by platelet integrin alphaIIbbold beta3

J. Gao, K.E. Zoller, M.H. Ginsberg, J.S. Brugge and S.J. Shattil

  • The EMBO Journal (1997) 16, 6414 - 6425
  • doi:10.1093/emboj/16.21.6414
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Tyrosine phosphorylation of proteins in CHO cells transfected with Syk. A5 cells were transfected with Syk (+ Syk) or a control plasmid (-Syk) and 48 h later incubated in suspension (BSA) or allowed to attach to fibrinogen (Fib) for 60 min. Total cell lysates (20 mug/lane) were then examined for tyrosine phosphorylation. The top arrow denotes a broad band at 120–130 kDa that increased in intensity with cell adhesion (lanes 1 versus 2 and 3 versus 4) The middle arrow denotes an unidentified approx80 kDa protein that was reproducibly dephosphorylated during cell adhesion (lanes 1 versus 2 and 3 versus 4) The bottom arrow denotes Syk (lane 4)

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