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Figure 2

Regulation of the pp72syk protein tyrosine kinase by platelet integrin alphaIIbbold beta3

J. Gao, K.E. Zoller, M.H. Ginsberg, J.S. Brugge and S.J. Shattil

  • The EMBO Journal (1997) 16, 6414 - 6425
  • doi:10.1093/emboj/16.21.6414
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Effect of CHO cell adhesion to ligands for alphaIIbbeta3 or beta1 integrins on tyrosine phosphorylation of Syk. A5 CHO cell transfectants were maintained in suspension for 60 min (BSA, lane 1) or allowed to attach to alphaIIbbeta3 ligands (fibrinogen, lane 2; anti-alphaIIbbeta3 monoclonal antibody D57, lane 3) or beta1 ligands (fibronectin, FN, lane 4; anti-beta1 monoclonal antibody 7E2, lane 5). Then tyrosine phosphorylation of Syk was assessed.

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