Table 1
A mutation mimicking ligand-induced conformational change yields a constitutive RXR that senses allosteric effects in heterodimers
Valerie Vivat, Christina Zechel, Jean-Marie Wurtz, William Bourguet, Hiroyuki Kagechika, Hiroki Umemiya, Koichi Shudo, Dino Moras, Hinrich Gronemeyer and Pierre Chambon
- The EMBO Journal (1997) 16, 5697 - 5709
- doi:10.1093/emboj/16.18.5697
| Receptor | Fold induction | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| DR1-tk | CRABPII | (TRE)3-tk | DR5-tk | ||||||
| – | 9-cis-RA | SR237 | – | 9-cis-RA | – | 9-cis-RA | – | SR237 | |
RXR wild-type | 1  0.1 | 26 2 | 26 0.5 | 1 0.1 | 40 3 | 1 0.1 | 11 1 | 0.8 0.1 | 13 1.5 |
| RXRF318A | 25 1 | 28 2 | 43 4.5 | 27 2 | 28 2 | 7.4 1.6 | 13 2 | 10 0.3 | 27 2 |
| The transient transactivation assays were performed in Cos1 cells; results are expressed as fold induction of reporter gene basal activity. Data are the mean SEM of three to six independent experiments. | |||||||||
