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| The EMBO Journal
(1997) 16, 5227–5234, doi:10.1093/emboj/16.17.5227 |
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| A new set of Mu DNA transposition intermediates: alternate pathways of target capture preceding strand transfer |
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| Darius Z. Naigamwalla and George Chaconas |
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Department of Biochemistry, University of Western Ontario, London, Ontario, Canada N6A 5C1 To whom correspondence should be addressed George Chaconas, chaconas@julian.uwo.ca Received 28 April 1997; Revised 25 June 1997. |
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| Abstract |
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| Mu DNA transposition occurs within the context of higher order nucleoprotein structures or transpososomes. We describe a new set of transpososomes in which Mu B-bound target DNA interacts non-covalently with previously characterized intermediates prior to the actual strand transfer. This interaction can occur at several points along the reaction pathway: with the LER, the Type 0 or the Type 1 complexes. The formation of these target capture complexes, which rapidly undergo the strand transfer chemistry, is the rate-limiting step in the overall reaction. These complexes provide alternate pathways to strand transfer, thereby maximizing transposition potential. This versatility is in contrast to other characterized transposons, which normally capture target DNA only at a single point in their respective reaction pathways. |
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| Keywords: DNA transposition, strand transfer, target complexes, transpososomes |
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