FIGURE 2
FROM:
-Globin mutation detection by tagged single-base extension and hybridization to universal glass and flow-through microarrays
Coline H M van Moorsel, Erwin E van Wijngaarden, Ivo F A C Fokkema, Johan T den Dunnen, Dirk Roos, Rob van Zwieten, Piero C Giordano and Cornelis L Harteveld
BACK TO ARTICLEFigure 2.
(a) Position of the 19 most frequent mutations within the Dutch population in the 
-globin gene and the position of the PCR primers. Introns in the -globin gene are called intervening sequences (IVS). The following primers were used: forward FF 5'-TGAAGTCCAACTCCTAAGCCA, reverse ER 5'-AAACGATCCTGAGACTTCCA, forward CF 5'-GTGTACACATATTGACCAAATC and reverse Q 5'-CCAAGGTTTGAACTAGCTCTTCA. (b) Secondary structure of the IVS2-1 primer. The first 20 5'-nucleotides represent the tag, and the last 23 nucleotides are mutation specific. Five consecutive bases at the 3' terminus are paired. The folded primer incorporates a labeled ddATP in the SBE reaction. The arrows indicate the site of the base substitution applied to prevent self-annealing of the 3' terminus.
