Short Report
European Journal of Human Genetics (2003) 11, 879–883. doi:10.1038/sj.ejhg.5201067
Karyotyping of human synaptonemal complexes by cenM-FISH
Maria Oliver-Bonet1, Thomas Liehr2, Angela Nietzel2, Anita Heller2, Heike Starke2, Uwe Claussen2, Montserrat Codina-Pascual1, Aïda Pujol1, Carlos Abad3, Josep Egozcue1, Joaquima Navarro1 and Jordi Benet1
- 1Unitat de Biologia, Facultat de Medicina, Departament de Biologia Cel
lular, Fisiologia i d'Immunologia, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain - 2Institute of Human Genetics and Anthropology, 07740 Jena, Germany
- 3Consorci Hospitalari Parc Taulí, 08208, Sabadell, Spain
Correspondence: Dr M Oliver-Bonet, Unitat de Biologia, Facultat de Medicina, Departament de Biologia Cellular, Fisiologia i d'Immunologia, Universitat Autònoma de Barcelona, Bellaterra 08193, Spain. Tel: +34 93 581 1175; Fax: +34 93 581 1025; E-mail: moliver@servet.uab.es
Received 24 March 2003; Revised 28 May 2003; Accepted 10 June 2003.
Abstract
The purpose of this work was to adapt the recently described centromere-specific multicolour (cenM-) FISH technique to human meiotic cells, and evaluate the usefulness of this multiplex fluorescence method for karyotyping human synaptonemal complex (SC), previously analysed by immunocytogenetic approaches. The results obtained demonstrate that cenM-FISH is a reliable one-single-step method, which allows for the identification of all SC present in pachytene spreads. Moreover, when cenM-FISH is applied after immunocytogenetic analysis, the number and distribution of MLH1 foci per chromosome can be established and recombination analysis for each chromosome can be performed easily.
Keywords:
cenM-FISH on synaptonemal complexes, immunocytogenetics, meiotic recombination
