Short Communication
European Journal of Clinical Nutrition (2009) 63, 446–449; doi:10.1038/ejcn.2008.29; published online 9 April 2008
Presence of 25(OH)D deficiency and its effect on vitamin D receptor mRNA expression
Contributors: All the authors contributed to the design of the study. RG wrote the manuscript and performed VDR mRNA expression studies and biochemical estimations and supervised the study. PC standardized the real time expression studies. MM and NT performed the real time PCR assay. DR extracted the RNA from duodenal tissue and performed biochemical assays including calcium estimation. NG performed the assay for 25(OH)D and intact PTH. VS analyzed the study data.
R Goswami1, A M Mondal2, N Tomar1, D Ray1, P Chattopadhyay2, N Gupta1 and V Sreenivas3
- 1Departments of Endocrinology and Metabolism, All India Institute of Medical Sciences, New Delhi, India
- 2Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India
- 3Department of Biostatistics, All India Institute of Medical Sciences, New Delhi, India
Correspondence: Dr R Goswami, Department of Endocrinology and Metabolism, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India. E-mail: gosravinder@hotmail.com
Received 4 January 2008; Revised 7 February 2008; Accepted 28 February 2008; Published online 9 April 2008.
Abstract
Background and objectives:
Vitamin D and its metabolites act through vitamin D receptor (VDR). We hypothesized that subjects with low serum 25(OH)D levels but normal PTH might have increased VDR expression.
Design and Methods:
VDRmRNA expression was assessed by real time PCR in duodenal mucosa and PBMC (peripheral blood mononuclear cells) in 45 subjects with normal duodenoscopy and in PBMC alone in 48 healthy volunteers with hypovitaminosis D. 25(OH)D, PTH and VDRmRNA expression in PBMC was reassessed after 8 weeks of oral cholecalciferol (60 000 IU per week) in a subset (n=23) of healthy volunteers.
Results and Conclusions:
The VDRmRNA expressions in the duodenum and PBMC were significantly correlated (r=0.42), but the expression was 13 times higher in the former than the latter. The mean VDRmRNA expression was similar in 25(OH)D-deficient subjects with or without PTH elevation, both in the duodenum and PBMC. The PBMC VDRmRNA expression showed no significant change after cholecalciferol supplementation. A weak correlation coefficient between duodenal mucosa and PBMC VDRmRNA suggests that caution needs to be exercised while using the latter as a surrogate for other sites.
Keywords:
25(OH)D deficiency, hypovitaminosis D, vitamin D receptor, mRNA expression; bioadaptation
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RESEARCH
Presence of 25(OH)D deficiency and its effect on vitamin D receptor mRNA expression
European Journal of Clinical Nutrition Scientific Correspondence
