Original Article

European Journal of Clinical Nutrition (2008) 62, 1432–1439; doi:10.1038/sj.ejcn.1602882; published online 15 August 2007

The Pro12Ala polymorphism of the PPAR-bold italic gamma2 gene affects associations of fish intake and marine n-3 fatty acids with glucose metabolism

Guarantor: SK Ylönen.

Contributors: AA, LG, SMV and SKY planned the study. SMV and SKY were responsible for dietary data collection; VL, LG and CS for the collection of the other data and IS supervised plasma fatty acid analyses. SKY conducted the data analyses and wrote the first draft of the manuscript. All authors were involved in the interpretation of the results and the revising of the manuscript.

S K Ylönen1,2, I Salminen3, V Lyssenko4, S M Virtanen5,6,7, L Groop4, A Aro3, C Saloranta2,8 and the Botnia Research Group

  1. 1Department of Applied Chemistry and Microbiology, Division of Nutrition, University of Helsinki, Helsinki, Finland
  2. 2Folkhälsan Research Center, Institute of Genetics, Helsinki, Finland
  3. 3Department of Health and Functional Capacity, National Public Health Institute, Helsinki, Finland
  4. 4Department of Endocrinology, University of Lund, Malmö, Sweden
  5. 5Department of Epidemiology and Health Promotion, National Public Health Institute, Helsinki, Finland
  6. 6Research Unit, Tampere University Hospital, Tampere, Finland
  7. 7Tampere School of Public Health, University of Tampere, Tampere, Finland
  8. 8Department of Medicine, Division of Diabetology, Helsinki University Hospital, Helsinki, Finland

Correspondence: Dr SK Ylönen, Department of Applied Chemistry and Microbiology, Division of Nutrition, University of Helsinki, PO Box 66, Helsinki, FI-00014, Finland. E-mail: katriina.ylonen@helsinki.fi

Received 30 January 2006; Revised 3 April 2007; Accepted 30 April 2007; Published online 15 August 2007.

Top

Abstract

Background/Objectives:

 

Data on associations between marine n-3 fatty acids and glucose metabolism are inconsistent. Therefore, we explored effects of the Pro12Ala polymorphism in peroxisome proliferator-activated receptor (PPAR)-italic gamma2 gene on associations of fish intake and dietary and plasma eicosapentaenoic and docosahexaenoic acid with glucose metabolism. The design comprises of the cross-sectional analysis.

Subjects/Methods:

 

The Pro12Ala variant in the PPAR-italic gamma2 (PPARG) gene was genotyped in 571 non-diabetic relatives of subjects with type II diabetes. The dietary intake was measured by a 3-day food record, and the plasma cholesterol ester fatty acid composition was analysed with gas chromatography. Associations of dietary and plasma variables with insulin resistance and fasting and 2-h glucose and free fatty acid concentrations were analysed with multiple linear regression analysis.

Results:

 

In men, there was a significant interaction between PPARG polymorphism and plasma docosahexaenoic acid on fasting free fatty acid concentration (P=0.036), and genotype-stratified models showed an inverse association in Pro homozygotes only (P=0.028). In women, the proportion of plasma eicosapentaenoic acid was higher in Ala-allele carriers compared to Pro homozygotes (1.67 vs 1.44% respectively, P=0.006). A significant interaction between PPARG polymorphism and fish intake on 2-h glucose was found in women (P=0.021), and genotype-stratified models suggested an inverse association in Ala-allele carriers only (P=0.039).

Conclusions:

 

The findings suggest that PPARG polymorphism might affect the plasma proportion of eicosapentaenoic acid and modulate the associations of fish intake and marine n-3 fatty acids with glucose metabolism and fasting free fatty acids.

Keywords:

fish intake, marine n-3 fatty acids, PPAR-italic gamma2 Pro12Ala polymorphism, insulin resistance, glucose concentrations, FFA concentrations

Extra navigation

.

naturejobs

More science jobs
Post a job for free
ADVERTISEMENT