Original Communication

European Journal of Clinical Nutrition (2005) 59, 393–398. doi:10.1038/sj.ejcn.1602086 Published online 17 November 2004

High intakes of milk, but not meat, increase s-insulin and insulin resistance in 8-year-old boys

Guarantor: C Hoppe.

Contributors: CH conducted the statistical analyses and prepared the first draft of the manuscript in collaboration with CM, AV and KFM. VB was responsible for all measurements of amino acids. All contributors participated in interpreting the results and were involved in preparing the final draft of the manuscript. No author had a financial or personal conflict of interest related to this research or its source of funding.

C Hoppe1, C Mølgaard1, A Vaag2, V Barkholt3 and K F Michaelsen1

  1. 1Department of Human Nutrition and Centre for Advanced Food Studies, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark
  2. 2Steno Diabetes Centre, Gentofte, Denmark
  3. 3BioCentrum-DTU, Biochemistry and Nutrition and Centre for Advanced Food Studies, Technical University of Denmark, Lyngby, Denmark

Correspondence: C Hoppe, Department of Human Nutrition and Centre for Advanced Food Studies, The Royal Veterinary and Agricultural University, Rolighedsvej 30, DK-1958 Frederiksberg, Denmark. E-mail: cho@kvl.dk

Received 30 March 2004; Revised 10 August 2004; Accepted 6 September 2004; Published online 17 November 2004.





Our objective was to examine if a high animal protein intake from milk or meat increased s-insulin and insulin resistance in healthy, prepubertal children. A high animal protein intake results in higher serum branched chain amino acids (BCAA; leucine, isoleucine and valine) concentrations, which are suggested to stimulate insulin secretion. Furthermore, milk possesses some postprandial insulinotrophic effect that is not related to its carbohydrate content.



A total of 24 8-y-old boys were asked to take 53 g protein as milk or meat daily. At baseline and after 7 days, diet was registered, and insulin, glucose, and amino acids were determined. Insulin resistance and beta cell function were calculated with the homeostasis model assessment.



Protein intake increased by 61 and 54% in the milk- and meat-group, respectively. In the milk-group, fasting s-insulin concentrations doubled, which caused the insulin resistance to increase similarly. In the meat-group, there was no increase in insulin and insulin resistance. As the BCAAs increased similarly in both groups, stimulation of insulin secretion through BCAAs is not supported.



Our results indicate that a short-term high milk, but not meat, intake increased insulin secretion and resistance. The long-term consequences of this are unknown. The effect of high protein intakes from different sources on glucose–insulin metabolism needs further studying.


dietary protein, children, insulin, insulin resistance, milk, meat, HOMA



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