Original Communication
European Journal of Clinical Nutrition (2005) 59, 24–34. doi:10.1038/sj.ejcn.1602028 Published online 11 August 2004
Effect of dietary fatty acids on the postprandial fatty acid composition of triacylglycerol-rich lipoproteins in healthy male subjects
Guarantors: A Bysted and G Hølmer.
Contributors: TT, BS, GH, and AB were involved in designing the study. AB analysed all blood samples and prepared chylomicron fractions for determination of the positional distributions. AB performed the statistical calculations and analyses, wrote the original manuscript and edited all subsequent versions. GH and PL supervised the analyses. GH contributed to the original and subsequent manuscripts. TT and BS performed the human trial, prepared lipoprotein fractions for fatty acid analyses, and revised the manuscript.
A Bysted1, G Hølmer1, P Lund1, B Sandström2,
and T Tholstrup2
- 1BioCentrum-DTU, Section of Biochemistry and Nutrition, Center of Advanced Food Studies, The Technical University of Denmark, Lyngby, Denmark
- 2Research Department of Human Nutrition, Center of Advanced Food Studies, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark
Correspondence: A Bysted, Department of Food Chemistry, Danish Institute for Food and Veterinary Research, Moerkhoej Bygade 19, DK-2860 Soeborg, Denmark. E-mail: aby@dfvf.dk
Deceased.
Received 10 July 2003; Revised 18 May 2004; Accepted 2 June 2004; Published online 11 August 2004.
Abstract
Objective: The aim of the present study was to investigate the effect of trans-18:1 isomers compared to other fatty acids, especially saturates, on the postprandial fatty acid composition of triacylglycerols (TAG) in chylomicrons and VLDL.
Design: A randomised crossover experiment where five interesterified test fats with equal amounts of palmitic acid (P fat), stearic acid (S fat), trans-18:1 isomers (T fat), oleic acid (O fat), or linoleic acid (L fat) were tested.
Subjects: A total of 16 healthy, normolipidaemic males (age 23
2 y) were recruited.
Interventions: The participants ingested fat-rich test meals (1 g fat per kg body weight) and the fatty acid profiles of chylomicron and VLDL TAG were followed for 8 h.
Results: The postprandial fatty acid composition of chylomicron TAG resembled that of the ingested fats. The fatty acids in chylomicron TAG were randomly distributed among the three positions in accordance with the distributions in test fats. Calculations of postprandial TAG concentrations from fatty acid data revealed increasing amounts up to 4 h but lower response curves (IAUC) for the two saturated fats in accordance with previous published data. The T fat gave results comparable to the O and L fats. The test fatty acids were much less reflected in VLDL TAG and there was no dietary influence on the response curves.
Conclusions: The fatty acid composition in the test fats as well as the positional distributions of these were maintained in the chylomicrons. No specific clearing of chylomicron TAG was observed in relation to time.
Sponsorship: Danish Research Development Program for Food Technology.
Keywords:
trans-fatty acids, saturated fatty acids, postprandial, triacylglycerol-rich lipoproteins, absorption, clearing
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