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Consumption of black currants, lingonberries and bilberries increases serum quercetin concentrations

European Journal of Clinical Nutrition volume 57, pages 37–42 (2003)Cite this article

Abstract

Objective: To study serum quercetin concentrations of subjects consuming berries or habitual Finnish diets.

Design: Randomized parallel dietary intervention.

Subjects: Forty healthy men (age 60 y).

Intervention: Twenty subjects consumed 100 g/day of berries (black currants, lingonberries and bilberries) for 8 weeks. Twenty subjects consuming their habitual diets served as controls. Fasting blood samples were obtained 2 weeks prior to the study, at baseline, and at 2, 4 and 8 weeks. Intake of quercetin was assessed from 3 day food records collected at baseline and at 8 weeks.

Results: The serum quercetin concentrations were significantly higher in the subjects consuming berries compared to the control group (P=0.039 ANCOVA with repeated measures). During the berry consumption period the mean serum concentrations of quercetin ranged between 21.4 and 25.3 µg/l in the berry group, which was 32–51% higher compared with the control group. According to 3 day food records, there was no difference in quercetin intake at baseline, but at 8 weeks the intake was 12.3±1.4 mg/day (mean±s.e.m.) in the berry group and 5.8±0.6 mg/day in the control group (P=0.001).

Conclusion: The results indicate that the berries used in this study are a good source of bioavailable quercetin.

Sponsorship: The study was supported by the Academy of Finland, Juho Vainio Foundation and the Finnish Foundation for Cardiovascular Research.

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References

  • Aviram, M & Fuhrman, B (1998). Polyphenolic flavonoids inhibit macrophage-mediated oxidation of LDL and attenuate atherogenesis. Atherosclerosis, 137, (Suppl) S45–50.

    Article  CAS  Google Scholar 

  • Caltagirone, S, Ranelletti, FO, Rinelli, A, Maggiano, N, Colasante, A, Musiani, P, Aiello, FB & Piantelli, M (1997). Interaction with type II estrogen binding sites and antiproliferative activity of tamoxifen and quercetin in human non-small-cell lung cancer. Am. J. Respir. Cell Mol. Biol., 17, 51–59.

    Article  CAS  Google Scholar 

  • Chopra, M, Fitzsimons, PE, Strain, JJ, Thurnham, DI & Howard, AN (2000). Nonalcoholic red wine extract and quercetin inhibit LDL oxidation without affecting plasma antioxidant vitamin and carotenoid concentrations. Clin. Chem., 46, 1162–1170.

    CAS  PubMed  Google Scholar 

  • de Vries, JH, Hollman, PC, van Amersfoort, I, Olthof, MR & Katan, MB (2001). Red wine is a poor source of bioavailable flavonols in men. J. Nutr., 131, 745–748.

    Article  CAS  Google Scholar 

  • Erlund, I, Alfthan, G, Siren, H, Ariniemi, K & Aro, A (1999). Validated method for the quantitation of quercetin from human plasma using HPLC with electrochemical detection. J. Chromatogr. B Biomed. Applic., 727, 179–189.

    Article  CAS  Google Scholar 

  • Erlund, I, Kosonen, T, Alfthan, G, Mäenpää, J, Perttunen, K, Kenraali, J, Parantainen, J & Aro, A (2000). Pharmacokinetics of quercetin from quercetin aglycone and rutin in healthy volunteers. Eur. J. Clin. Pharmac., 56, 545–553.

    Article  CAS  Google Scholar 

  • Erlund, I, Alfthan, G, Mäenpää, J & Aro, A (2001). Tea and coronary heart disease: the flavonoid quercetin is more bioavailable from rutin in women than in men. Arch. Intern. Med., 161, 1919–1920.

    Article  CAS  Google Scholar 

  • Erlund, I, Silaste, ML, Alfthan, G, Rantala, M, Kesäniemi, YA & Aro, A (2002). Plasma concentrations of the flavonoids naringenin, hesperetin and quercetin in human subjects following their habitual diets, or diets high or low in fruits and vegetables. Eur. J. Clin. Nutr., (in press)

  • Freese, R, Alfthan, G, Jauhiainen, M, Basu, S, Erlund, I, Salminen, I, Aro, A & Mutanen, M (2002). High intake of vegetables, berries and apple combined with high intake of linoleic or oleic acid only slightly affects markers of lipid peroxidation and lipoprotein metabolism in healthy subjects. Am. J. Clin. Nutr., (in press)

  • Hakala, P, Marniemi, J, Knuts, L-R, Kumpulainen, J, Tahvonen, R & Plaami, S (1996). Calculated vs. analysed nutrient composition of weight reduction diets. Food Chem., 57, 71–75.

    Article  CAS  Google Scholar 

  • Häkkinen, S & Auriola, S (1998). High-performance liquid chromatography with electrospray ionization mass spectrometry and diode array ultraviolet detection in the identification of flavonol aglycones and glycosides in berries. J. Chromatogr. A., 829, 91–100.

    Article  Google Scholar 

  • Häkkinen, SH, Kärenlampi, SO, Heinonen, IM, Mykkänen, HM & Törrönen, AR (1999). Content of the flavonols quercetin, myricetin, and kaempferol in 25 edible berries. J. Agric. Food Chem., 47, 2274–2279.

    Article  Google Scholar 

  • Häkkinen, S (2000). Flavonols and phenolic acids in berries and berry products (dissertation). Kuopio: University of Kuopio

  • Hertog, MGL, Kromhout, D, Aravanis, C, Blackburn, H, Buzina, R & Fidanza, F et al (1995). Flavonoid intake and long-term risk of coronary heart disease and cancer in the Seven Countries Study. Arch. Intern. Med., 155, 381–386.

    Article  CAS  Google Scholar 

  • Hirvonen, T (2001). Flavonol and flavone intake and risk of cardiovascular disease and cancer in male smokers (dissertation). Helsinki: Publications of the National Public Health Institute (KTL) A19/2001

  • Hollman, PC, van Trijp, JM, Buysman, MN, van der Gaag, MS, Mengelers, MJ, de Vries, J & Katan, MB (1997). Relative bioavailability of the antioxidant flavonoid quercetin from various foods in man. FEBS Lett., 418, 152–156.

    Article  CAS  Google Scholar 

  • Hollman, PC, Van Het Hof, KH, Tijburg, LB & Katan, MB (2001). Addition of milk does not affect the absorption of flavonols from tea in man. Free Radic. Res., 34, 297–300.

    Article  CAS  Google Scholar 

  • Johansson, L, Becker, W, Fagt, S, porgeirsdóttir, H & Valsta, L (1998). Grønnsak-og fruktinntakket i Norden (in Norwegian), Uppsala: Nordiska Ministerrådet

    Google Scholar 

  • Kleemola, P, Virtanen, M & Pietinen, P (1994). The 1992 dietary survey of Finnish adults, Helsinki: Publications of the National Public Health Institute B2/1994

    Google Scholar 

  • Knekt, P, Jarvinen, R, Reunanen, A & Maatela, J (1996). Flavonoid intake and coronary mortality in Finland: a cohort study. Br. Med. J., 312, 478–481.

    Article  CAS  Google Scholar 

  • Knuts, L-R, Rastas, M, Haapala, P, Seppänen, R, Karvetti, R-L & Aro, A (1987). Nutrica–a computer program for calculation of food and nutrient intake. Abstracts of the Third Meeting of Eurofoods, Warsaw, 24–27 May 1987, p16

  • Koeppen, BH & Herrmann, K (1977). Flavonoid glycosides and hydroxycinnamic acid esters of blackcurrants (Ribes nigrum). Phenolics of fruits 9. Z Lebensm Unters Forsch., 164, 263–268.

    Article  CAS  Google Scholar 

  • Kühnau, J (1976). The flavonoids. A class of semi-essential food components: their role in human nutrition. World Rev. Nutr. Diet, 24, 117–191.

    Article  Google Scholar 

  • Ludden, TM (1991). Nonlinear pharmocokinetics. Clinical implications. Clin. Pharmacokinet., 20, 429–446.

    Article  CAS  Google Scholar 

  • Marniemi, J, Hakala, P, Mäki, J & Ahotupa, M (2000). Partial resistance of low density lipoprotein to oxidation in vivo after increased intake of berries. Nutr. Metab. Cardiovasc. Dis., 10, 331–337.

    CAS  PubMed  Google Scholar 

  • Mattila, P, Astola, J & Kumpulainen, J (2000). Determination of flavonoids in plant material by HPLC with diode-array and electro-array detections. J. Agric. Food Chem., 48, 5834–5841.

    Article  CAS  Google Scholar 

  • Mikkonen, TP, Määttä, KR, Hukkanen, AT, Kokko, HI, Törrönen, AR, Kärenlampi, SO & Karjalainen, RO (2001). Flavonol content varies among black currant cultivars. J. Agric. Food Chem., 49, 3274–3277.

    Article  CAS  Google Scholar 

  • Murkovic, M, Adam, U & Pfannhauser, W (2000). Analysis of anthocyane glycosides in human serum. Fresenius J. Anal. Chem., 366, 379–381.

    Article  CAS  Google Scholar 

  • Noroozi, M, Burns, J, Crozier, A, Kelly, IE & Lean, ME (2000). Prediction of dietary flavonol consumption from fasting plasma concentration or urinary excretion. Eur. J. Clin. Nutr., 54, 143–149.

    Article  CAS  Google Scholar 

  • Peet, GW & Li, J (1999). IkappaB kinases alpha and beta show a random sequential kinetic mechanism and are inhibited by staurosporine and quercetin. J. Biol. Chem., 274, 32655–32661.

    Article  CAS  Google Scholar 

  • Pereira, MA, Grubbs, CJ, Barnes, LH, Li, H, Olson, GR, Eto, I, Juliana, M, Whitaker, LM, Kelloff, GJ, Steele, VE & Lubet, RA (1996). Effects of the phytochemicals, curcumin and quercetin, upon azoxymethane-induced colon cancer and 7, 12-dimethylbenz[a]anthracene-induced mammary cancer in rats. Carcinogenesis, 17, 1305–1311.

    Article  CAS  Google Scholar 

  • Siess, MH, Leclerc, J, Canivenc-Lavier, MC, Rat, P & Suschetet, M (1995). Heterogenous effects of natural flavonoids on monooxygenase activities in human and rat liver microsomes. Toxicol. Appl. Pharmac., 130, 73–78.

    Article  CAS  Google Scholar 

  • Yochum, L, Kushi, LH, Meyer, K & Folsom, AR (1999). Dietary flavonoid intake and risk of cardiovascular disease in postmenopausal women. Am. J. Epidemiol., 149, 943–949.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We would like to thank Dr M-L Ovaskainen for providing data on quercetin concentrations of foods and for calculating berry consumption in the 1992 Dietary Survey of Finnish Adults.

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Author notes

  1. J Marniemi and A Aro: Guarantors: AA and JM

  2. I Erlund, J Marniemi, P Hakala, G Alfthan, E Meririnne and A Aro: Contributors: IE performed quercetin analyses, interpreted the data and wrote the article. PH and JM designed and executed the dietary intervention. PH calculated quercetin intake. EM performed statistical analyses. GA and AA supervised quercetin analyses. All authors read and commented on the manuscript.

Authors and Affiliations

  1. Biomarker Laboratory, National Public Health Institute, Helsinki, Finland

    I Erlund, G Alfthan & A Aro

  2. Research and Development Centre, The Social Insurance Institution, Turku, Finland

    J Marniemi & P Hakala

  3. Research Unit of Substance Abuse, National Public Health Institute, Helsinki, Finland

    E Meririnne

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  1. I Erlund
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Correspondence to I Erlund.

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Erlund, I., Marniemi, J., Hakala, P. et al. Consumption of black currants, lingonberries and bilberries increases serum quercetin concentrations. Eur J Clin Nutr 57, 37–42 (2003). https://doi.org/10.1038/sj.ejcn.1601513

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