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| Subject Categories: Cell & Tissue Architecture | Microbiology & Pathogens |
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| The EMBO Journal
(2002) 21, 5331–5342, doi: 10.1093/emboj/cdf550 |
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| Functions of LIM proteins in cell polarity and chemotactic motility |
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| Bharat Khurana2, 4, Taruna Khurana3, 4, Nandkumar Khaire1 and Angelika A. Noegel1 |
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1 Center for Biochemistry, Medical Faculty, University of Cologne, Joseph-Stelzmann-Strasse 52, D-50931 Cologne, Germany 2 Present address: Laboratory of Viral Diseases, Building 4, Room 131, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health, Bethesda, MD 20892-8028, USA 3 Present address: Laboratory of Cellular and Developmental Biology, Building 50, Room 3345, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health, Bethesda, MD 20892-8028, USA 4 B.Khurana and T.Khurana contributed equally to this work To whom correspondence should be addressed Angelika A. Noegel, noegel@uni-koeln.de Received 26 March 2002; Revised 5 August 2002; Accepted 28 August 2002. |
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| Abstract |
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| LimC and LimD are two novel LIM proteins of Dictyostelium, which are comprised of double and single LIM domains, respectively. Green fluorescent protein-fused LimC and LimD proteins preferentially accumulate at areas of the cell cortex where they co-localize with actin and associate transiently with cytoskeleton-dependent dynamic structures like phagosomes, macropinosomes and pseudopods. Furthermore, both LimC and LimD interact directly with F-actin in vitro. Mutant cells that lack either LimC or LimD, or both, exhibit normal growth. They are, however, significantly impaired in growth under stress conditions and are highly sensitive to osmotic shock, suggesting that LimC and LimD contribute towards the maintenance of cortical strength. Moreover, we noted an altered morphology and F-actin distribution in LimD- and LimC-/D- mutants, and changes in chemotactic motility associated with an increased pseudopod formation. Our results reveal both unique and overlapping roles for LimC and LimD, and suggest that both act directly on the actin cytoskeleton and provide rigidity to the cortex. |
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| Keywords: actin binding protein, cytoskeleton, mutants, pseudopod formation |
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