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| The EMBO Journal
(2001) 20, 5703–5714, doi:10.1093/emboj/20.20.5703 |
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| The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins |
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| Tobias C. Walther1, Maarten Fornerod1, 2, Helen Pickersgill3, Martin Goldberg3, Terry D. Allen3 and Iain W. Mattaj1 |
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1 European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany 2 Present address: Netherlands Cancer Institute – H4, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands 3 CRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital National Health Service Trust, Manchester M20 9BX, UK To whom correspondence should be addressed Iain W. Mattaj, mattaj@embl-heidelberg.de Received 22 June 2001; Revised 22 August 2001; Accepted 23 August 2001. |
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| Abstract |
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The nuclear pore complex (NPC) is a large proteinaceous structure through which bidirectional transport of macromolecules across the nuclear envelope (NE) takes place. Nup153 is a peripheral NPC component that has been implicated in protein and RNP transport and in the interaction of NPCs with the nuclear lamina. Here, Nup153 is localized by immunogold electron microscopy to a position on the nuclear ring of the NPC. Nuclear reconstitution is used to investigate the role of Nup153 in nucleo- cytoplasmic transport and NPC architecture. NPCs assembled in the absence of Nup153 lacked several nuclear basket components, were unevenly distributed in the NE and, unlike wild-type NPCs, were mobile within the NE. Importin  / -mediated protein import into the nucleus was strongly reduced in the absence of Nup153, while transportin-mediated import was unaffected. This was due to a reduction in import complex translocation rather than to defective receptor recycling. Our results therefore reveal functions for Nup153 in NPC assembly, in anchoring NPCs within the NE and in mediating specific nuclear import events. |
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| Keywords: NPC, nuclear basket, nuclear import, nuclear pore complex Nup153 |
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