|
 |
|
|
| The EMBO Journal
(1997) 16, 2452–2462, doi:10.1093/emboj/16.9.2452 |
|
| Multiple functions of Drosophila heat shock transcription factor in vivo |
|
|
| Paul Jedlicka2, Mark A. Mortin1 and Carl Wu2 |
|
|
1 Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Building 37, Room 4C-09, Bethesda, MD 20892-4255, USA 2 Present address: Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Building 37, Room 5E-26, Bethesda, MD 20892-4255, USA To whom correspondence should be addressed Carl Wu, carlwu@helix.nih.gov Received 21 November 1996; Revised 31 December 1996. |
|
|
|
| Abstract |
|
| Heat shock transcription factor (HSF) is a transcriptional activator of heat shock protein (hsp) genes in eukaryotes. In order to elucidate the physiological functions of HSF in Drosophila, we have isolated lethal mutations in the hsf gene. Using a conditional allele, we show that HSF has an essential role in the ability of the organism to survive extreme heat stress. In contrast to previous results obtained with yeast HSF, the Drosophila protein is dispensable for general cell growth or viability. However, it is required under normal growth conditions for oogenesis and early larval development. These two developmental functions of Drosophila HSF are genetically separable and appear not to be mediated through the induction of HSPs, implicating a novel action of HSF that may be unrelated to its characteristic function as a stress-responsive transcriptional activator. |
|
| Keywords: development, heat shock, mutant, thermotolerance, transcription |
|
|
| |
| |
| |
 | Email link to a friend |
| |
 | Download PDF |
| |
 | Full text |
| |
| |
| |
 | Next article |
| |
 | Previous article |
| |
 | Table of contents |
| |
| |
 | Rights and permissions |
| |
 | Reprints |
| |
| |
| |
 Register for table of contents by email | |
| |
| |
| |