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Molecular cloning and characterization of STAMP2, an androgen-regulated six transmembrane protein that is overexpressed in prostate cancer

Oncogene volume 24, pages 4934–4945 (2005)Cite this article

Abstract

We have identified a novel gene, six transmembrane protein of prostate 2 (STAMP2), named for its high sequence similarity to the recently identified STAMP1 gene. STAMP2 displays a tissue-restricted expression with highest expression levels in placenta, lung, heart, and prostate and is predicted to code for a 459-amino acid six transmembrane protein. Using a form of STAMP2 labeled with green flourescent protein (GFP) in quantitative time-lapse and immunofluorescence confocal microscopy, we show that STAMP2 is primarily localized to the Golgi complex, trans-Golgi network, and the plasma membrane. STAMP2 also localizes to vesicular-tubular structures in the cytosol and colocalizes with the Early Endosome Antigen1 (EEA1) suggesting that it may be involved in the secretory/endocytic pathways. STAMP2 expression is exquisitely androgen regulated in the androgen-sensitive, androgen receptor-positive prostate cancer cell line LNCaP, but not in androgen receptor-negative prostate cancer cell lines PC-3 and DU145. Analysis of STAMP2 expression in matched normal and tumor samples microdissected from prostate cancer specimens indicates that STAMP2 is overexpressed in prostate cancer cells compared with normal prostate epithelial cells. Furthermore, ectopic expression of STAMP2 in prostate cancer cells significantly increases cell growth and colony formation suggesting that STAMP2 may have a role in cell proliferation. Taken together, these data suggest that STAMP2 may contribute to the normal biology of the prostate cell, as well as prostate cancer progression.

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Acknowledgements

This work was supported by grants from the Norwegian Research Council, Norwegian Cancer Society, and University of Oslo to FS. We thank Dr Erik Snapp and members of the FS lab for helpful discussions.

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Author notes

  1. Ceren G Korkmaz, Kemal S Korkmaz and Piotr Kurys: These authors contributed equally to this work

Authors and Affiliations

  1. Department of Molecular Biosciences, University of Oslo, Postboks 1050 Blindern, 0316, Oslo, Norway

    Ceren G Korkmaz, Kemal S Korkmaz, Piotr Kurys, Ling Wang, Tove I Klokk & Fahri Saatcioglu

  2. Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, 20892, MD, USA

    Cem Elbi & Gordon L Hager

  3. Department of Pathology, Aker University Hospital, Oslo, Norway

    Clara Hammarstrom & Aud Svindland

  4. Department of Pathology, The Norwegian Radium Hospital, Oslo, Norway

    Gunhild Troen

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  1. Ceren G Korkmaz
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Correspondence to Fahri Saatcioglu.

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Korkmaz, C., Korkmaz, K., Kurys, P. et al. Molecular cloning and characterization of STAMP2, an androgen-regulated six transmembrane protein that is overexpressed in prostate cancer. Oncogene 24, 4934–4945 (2005). https://doi.org/10.1038/sj.onc.1208677

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