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  • Oncogenomics
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Comparison of gene expression profiling between malignant and normal plasma cells with oligonucleotide arrays

Oncogene volume 21, pages 6848–6857 (2002)Cite this article

Abstract

The DNA microarray technology enables the identification of the large number of genes involved in the complex deregulation of cell homeostasis taking place in cancer. Using Affymetrix microarrays, we have compared the gene expression profiles of highly purified malignant plasma cells from nine patients with multiple myeloma (MM) and eight myeloma cell lines to those of highly purified nonmalignant plasma cells (eight samples) obtained by in vitro differentiation of peripheral blood B cells. Two unsupervised clustering algorithms classified these 25 samples into two distinct clusters: a malignant plasma cell cluster and a normal plasma cell cluster. Two hundred and fifty genes were significantly up-regulated and 159 down-regulated in malignant plasma samples compared to normal plasma samples. For some of these genes, an overexpression or downregulation of the encoded protein was confirmed (cyclin D1, c-myc, BMI-1, cystatin c, SPARC, RB). Two genes overexpressed in myeloma cells (ABL and cystathionine beta synthase) code for enzymes that could be a therapeutic target with specific drugs. These data provide a new insight into the understanding of myeloma disease and prefigure that the development of DNA microarray could help to develop an ‘à la carte’ treatment in cancer disease.

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Acknowledgements

We thank Eric Legouffe for providing the myeloma samples and Linda Northrup for manuscript correction. This work was supported by grants from the Ligue Nationale Contre le Cancer, Paris, France.

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Authors and Affiliations

  1. INSERM U475, CHU Montpellier, 34 000, France

    John De Vos, Karin Tarte, Matthias Ensslen, Pierre Raynaud, Florence Pellet, Véronique Pantesco, Thierry Rème, Michel Jourdan & Bernard Klein

  2. Service d'Hématologie et d'Oncologie Médicale CHU Montpellier, 34 000, France

    Jean-François Rossi

  3. Unit for Cellular Therapy, CHU Montpellier, 34 000, France

    John De Vos, Guilhem Requirand & Bernard Klein

  4. Department of Clinical Biochemistry, Molecular Diagnostic Laboratory, Brendstrupgaardsvej, Aarhus N, 8200, Denmark

    Thomas Thykjær & Torben Ørntoft

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  1. John De Vos
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Correspondence to Bernard Klein.

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De Vos, J., Thykjær, T., Tarte, K. et al. Comparison of gene expression profiling between malignant and normal plasma cells with oligonucleotide arrays. Oncogene 21, 6848–6857 (2002). https://doi.org/10.1038/sj.onc.1205868

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