Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

A translation repressor element resides in the 3′ untranslated region of human p53 mRNA

Oncogene volume 18, pages 6419–6424 (1999)Cite this article

Abstract

The 3′ untranslated region of human p53 mRNA represses translation both in vitro and in vivo. Here, we identify a cis-acting 66-nucleotide U-rich sequence in the human p53 mRNA 3′ untranslated region that mediates translational repression. Using UV cross-linking, we detect a 40 kDa protein that interacts specifically with the p53 3′UTR containing the repressor element. Enhanced translation of p53 mRNA contributes to the accumulation of p53 protein in cells exposed to γ-radiation and could be a consequence of relieving the inhibition mediated by the repressor element.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3

Similar content being viewed by others

References

  • Bashaw GJ and Baker SB. . 1997 Cell 89: 789–798.

  • Baum EZ, Hyman LE and Wormington MW. . 1988 Dev. Biol. 126: 141–149.

  • Bradford MM. . 1976 Anal. Biochem. 72: 248–258.

  • Chirgwin JM, Przybyla AE, McDonald RJ and Rutter WJ. . 1979 Biochemistry 18: 5294–5299.

  • Clarke AR, Purdie CA, Harrison DJ, Morris RG, Bird CC, Hooper ML and Wyllie AH. . 1993 Nature 362: 849–852.

  • de Wet JR, Wood KV, Deluca M, Helinski DR and Subramani S. . 1987 Mol. Cell. Biol. 7: 725–737.

  • Fort P, Marty L, Piechaczyk M, Sabrouby SE, Dani C, Jeanteur P and Blanchard JM. . 1985 Nucleic Acids Res. 13: 1431–1442.

  • Fritsche M, Haessler C and Brandner G. . 1993 Oncogene 8: 307–318.

  • Fu L, Minden MD and Benchimol S. . 1996 EMBO J. 15: 4392–4401.

  • Fu L and Benchimol S. . 1997 EMBO J. 16: 4117–4125.

  • Giaccia AJ and Kastan MB. . 1998 Genes Dev. 12: 2973–2983.

  • Hake LE and Richter JD. . 1994 Cell 79: 617–627.

  • Harlow E, Williamson NM, Ralston R, Helfman DM and Adams TE. . 1985 Mol. Cell. Biol. 5: 1601–1610.

  • Herzog H, Hofferer L, Schneider R and Schweiger M. . 1990 Nucleic Acids Res. 18: 4600.

  • Jackson RJ and Standart N. . 1993 Cell 62: 15–24.

  • Kastan MB, Onyekwere O, Sidransky D, Vogelstein B and Craig RW. . 1991 Cancer Res. 51: 6304–6311.

  • Kuerbitz SJ, Plunkett BS, Walsh WV and Kastan MB. . 1992 Proc. Natl. Acad. Sci. USA 89: 7491–7495.

  • Lowe SW, Ruley HE, Jacks T and Housman DE. . 1993a Cell 74: 957–967.

  • Lowe SW, Schmitt EM, Smith SW, Osborne BA and Jacks T. . 1993b Nature 362: 847–849.

  • Lu X and Lane DP. . 1993 Cell 75: 765–778.

  • Maki CG and Howley PM. . 1997 Mol. Cell. Biol. 17: 355–363.

  • Maltzman W and Czyzyk L. . 1984 Mol. Cell. Biol. 4: 1689–1694.

  • Matlashewski G, Lamb P, Pim D, Peacock J, Crawford L and Benchimol S. . 1984 EMBO J. 33: 3257–3262.

  • Melton DA, Krieg P, Rebagliati M, Maniatis T, Sinn K and Green M. . 1984 Nucleic Acids Res. 12: 7035–7056.

  • Mosner J, Mummenbrauer T, Bauer C, Sczakiel G, Grosse F and Deppert W. . 1995 EMBO J. 14: 442–449.

  • Murata Y and Wharton RP. . 1995 Cell 80: 747–756.

  • Oren M, Maltzman W and Levine AJ. . 1981 Mol. Cell. Biol. 1: 101–110.

  • Ostareck DH, Ostareck-Lederer A, Wilm M, Thiele BJ, Mann M and Hentze MW. . 1997 Cell 89: 597–606.

  • Pennica D, Goeddel DV, Hayflick JS, Reich NC, Anderson CW and Levine AJ. . 1984 Virology 134: 477–482.

  • Rogel A, Popliker M, Webb CG and Oren M. . 1985 Mol. Cell. Biol. 5: 2851–2855.

  • Standart N and Jackson RJ. . 1994 Biochimie 76: 867–879.

  • Tishler RB, Calderwood SK, Coleman CN and Price BD. . 1993 Cancer Res. 53: 2212–2216.

  • Wickens M, Anderson MP and Jackson RJ. . 1997 Curr. Opin. Genet. Dev. 7: 220–232.

Download references

Acknowledgements

We thank Michael Wormington for providing us with the pSP65AT plasmid, and Barbara Stebbins-Boaz and Joel Richter for suggestions and advice with the UV cross-linking assay. We also wish to thank Yunping Lin, Homayoun Vaziri and John Kim for critical reading of the manuscript. This work was supported by the National Cancer Institute of Canada and the Medical Research Council of Canada.

Author information

Authors and Affiliations

  1. Ontario Cancer Institute/Princess Margaret Hospital, 610 University Avenue, Toronto, Ontario, M5G 2M9, Canada

    Loning Fu, WeiLi Ma & Samuel Benchimol

  2. Department of Medical Biophysics, University of Toronto, 610 University Avenue, Toronto, Ontario, M5G 2M9, Canada

    Samuel Benchimol

Authors
  1. Loning Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. WeiLi Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Samuel Benchimol
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fu, L., Ma, W. & Benchimol, S. A translation repressor element resides in the 3′ untranslated region of human p53 mRNA. Oncogene 18, 6419–6424 (1999). https://doi.org/10.1038/sj.onc.1203064

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1203064

Keywords

This article is cited by

Search

Advanced search

Quick links