Abstract
Norovirus is increasingly recognized as a frequent cause of non-bacterial gastroenteritis. Despite a 10-fold increase in the number of cases reported following the availability of enzyme immunoassays, there are no reports yet from preterm neonates. We report on a sudden clustering of antigen-positive enzyme immuno assays results in a level III neonatal intensive care unit, involving 22 of 43 infants screened. Although antigen-positive samples were significantly associated with bloody stools (P<0.001) and gastric residues (P<0.02), norovirus infection could not be confirmed by reverse-transcriptase polymerase chain reaction or electron microscopy. We question the validity of the so called norovirus-specific antigen assays and warn against overreacting to positive enzyme immunoassays results without reverse-transcriptase polymerase chain reaction confirmation especially in the neonatal setting.
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We thank Dr. Schreier at the Robert Koch Institute, Berlin for providing data on RT-PCR.
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Wiechers, C., Bissinger, A., Hamprecht, K. et al. Apparently non-specific results found using a norovirus antigen immunoassay for fecal specimens from neonates. J Perinatol 28, 79–81 (2008). https://doi.org/10.1038/sj.jp.7211849
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DOI: https://doi.org/10.1038/sj.jp.7211849
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