Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Paper
  • Published:

ASP stimulates glucose transport in cultured human adipocytes

Abstract

OBJECTIVE: The purpose of the present study was to examine the effect of Acylation Stimulating Protein (ASP) on glucose transport in cultured subcutaneous adipocytes. DESIGN AND SUBJECTS: Subcutaneous adipose tissue was obtained from non-obese, healthy females (18–32 y old) undergoing mammoplasty reduction. Preadipocytes were isolated and differentiated into adipocytes. MEASUREMENTS: Following the exposure of preadipocytes and adipocytes to ASP or insulin, glucose transport was assessed as [3H] 2-deoxy glucose uptake. The measurements were normalised per total cell protein. RESULTS: ASP increases specific membrane glucose transport in both preadipocytes and adipocytes in a time and concentration dependent manner. Stimulation in both cell types is rapid (within minutes), reaching a maximal effect between 1 and 4 h. However, after 24 h exposure to ASP, there is a downregulation in the response. The ASP response is greater following differentiation of preadipocytes to adipocytes and is compared to that of insulin. Dose response studies demonstrated a five-fold greater sensitivity of adipocytes (half-maximal concentration of ASP on adipocytes=0.5 µM, preadipocytes=2.3 µM). CONCLUSION: These results demonstrate that ASP not only stimulates triglyceride synthesis, but also glucose transport in differentiated human adipocytes and is consistent with a physiologically important role for ASP in postprandial energy storage.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Maslowska, M., Sniderman, A., Germinario, R. et al. ASP stimulates glucose transport in cultured human adipocytes. Int J Obes 21, 261–266 (1997). https://doi.org/10.1038/sj.ijo.0800396

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.ijo.0800396

Keywords

This article is cited by

Search

Quick links