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SPECT/CT imaging of baculovirus biodistribution in rat

Gene Therapy volume 14, pages 930–938 (2007)Cite this article

Abstract

Non-invasive imaging provides essential information regarding the biodistribution of gene therapy vectors and it can also be used for the development of targeted vectors. In this study, we have utilized micro Single-photon emission computed tomography to visualize biodistribution of a 99mTc-polylys-ser-DTPA-biotin-labelled avidin-displaying baculovirus, Baavi, after intrafemoral (i.f.), intraperitoneal (i.p.), intramuscular (i.m.) and intracerebroventricular (i.c.v.) administration. The imaging results suggest that the virus can spread via the lymphatic network after different administration routes, also showing accumulation in the nasal area after systemic administration. Extensive expression in the kidneys and spleen was seen after i.p. administration, which was confirmed by reverse transcriptase-polymerase chain reaction and immunohistochemistry. Additionally, transduction of kidneys was seen with i.m. and i.f. administrations. We conclude that baculovirus may be beneficial for the treatment of kidney diseases after i.p. administration route.

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Acknowledgements

We thank the Finnish Academy (JH and SY), the Sigrid Juselius Foundation (JH and SY), Instrumentarium Science Foundation (JH and TL), Finnish Cultural Foundation (TL), Orion Science Foundation (TL) and Ark Therapeutics Ltd (JR, MK) for support. We also thank Ms Maarit Pulkkinen, Ms Tarja Taskinen, Ms Riikka Eisto and Mr Joonas Malinen for excellent technical assistance.

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Authors and Affiliations

  1. Department of Biotechnology and Molecular Medicine, AI Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland

    J K Räty, M U Kaikkonen, K J Airenne & S Ylä-Herttuala

  2. Ark Therapeutics Oy, Kuopio, Finland

    J K Räty, M U Kaikkonen & K J Airenne

  3. Department of Biomedical NMR, Cellular and Molecular Imaging Group, AI Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland

    T Liimatainen & J M Hakumäki

  4. Department of Chemistry, University of Kuopio, Kuopio, Finland

    T Huhtala & A Närvänen

  5. Department of Clinical Radiology, University of Kuopio, Kuopio, Finland

    J M Hakumäki

  6. Department of Medicine and Gene Therapy Unit, University of Kuopio, Kuopio, Finland

    S Ylä-Herttuala

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  1. J K Räty
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Correspondence to S Ylä-Herttuala.

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Räty, J., Liimatainen, T., Huhtala, T. et al. SPECT/CT imaging of baculovirus biodistribution in rat. Gene Ther 14, 930–938 (2007). https://doi.org/10.1038/sj.gt.3302934

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