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Adeno-associated virus type 4 (AAV4) targets ependyma and astrocytes in the subventricular zone and RMS

Gene Therapy volume 12, pages 1503–1508 (2005)Cite this article

Abstract

The subventricular zone (SVZ) is one of the neurogenic niches in the adult mammalian brain. The SVZ is of interest for studies on neurogenesis and stem cell therapy. Here, we report specific transduction of ependyma and/or astrocytes by recombinant adeno-associated virus type 4 (AAV4) viral vectors. AAV4 vectors encoding β-galactosidase or eGFP were injected into the lateral ventricles of neonatal and adult C57BL/6 mouse brains. In addition, SVZ injections were conducted on adult mice. AAV4 vectors show a characteristic transduction of the ependyma independent of delivery route. However, AAV4 virus injected into the SVZ targeted GFAP positive astrocytes forming the glial tube in the SVZ and rostral migratory stream (RMS). Our results introduce AAV4 as a new tool by which to manipulate glial cells in the RMS.

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Acknowledgements

We thank members of the Davidson Laboratory for critical discussion, Stephanie M Hughes for assisting with early studies, Colleen S Stein for reviewing the manuscript and Christine McLennan for manuscript preparation. This work was supported by the NIH (HD 33531) and the University of Iowa Graduate Program in Neuroscience (GL).

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Authors and Affiliations

  1. Program in Gene Therapy, University of Iowa, Iowa City, IA, USA

    G Liu, I H Martins, J A Chiorini & B L Davidson

  2. Department of Internal Medicine, University of Iowa, Iowa City, IA, USA

    G Liu, I H Martins & B L Davidson

  3. Department of Neurology, University of Iowa, Iowa City, IA, USA

    B L Davidson

  4. National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA

    J A Chiorini

  5. Department of Physiology & Biophysics, University of Iowa, Iowa City, IA, USA

    B L Davidson

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  1. G Liu
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Liu, G., Martins, I., Chiorini, J. et al. Adeno-associated virus type 4 (AAV4) targets ependyma and astrocytes in the subventricular zone and RMS. Gene Ther 12, 1503–1508 (2005). https://doi.org/10.1038/sj.gt.3302554

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  • DOI: https://doi.org/10.1038/sj.gt.3302554

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