Abstract
Several lines of evidence suggest that interferon (IFN)-α is effective in suppression of liver cirrhosis (LC) as well as hepatitis C virus (HCV) infection, which is a major cause of LC in Japan. However, IFN-α often causes systemic toxicity such as flu-like symptoms, which precludes the IFN-α dose escalation required for clinical efficacy. Since IFN-α is rapidly degraded in the blood circulation, only a small amount of subcutaneously injected IFN-α protein can reach the target organ, the liver. It is expected that on-site IFN-α production in the liver overcomes the limitation of the conventional parenteral IFN-α administration. An adenovirus vector expressing the rat IFN-α gene (AxCA-rIFN) was injected intravenously into rats with dimethylnitrosamine-induced LC. While the subcutaneous IFN-α protein injection led to a transient elevation of the cytokine both in the liver and serum, the vector-mediated IFN-α gene transduction induced a significant amount of IFN-α detected in the liver but not in the serum. The injection of AxCA-rIFN prevented the progression of the rat LC, and improved the survival rate of the treated rats. Although no significant toxicity was noted in the animals, we showed that IFN-α gene expression in the liver can be efficiently downregulated by the Cre/loxP-mediated shut-off system, in case the IFN-α overdose becomes a problem. The study suggested for the first time the advantage and feasibility of IFN-α gene therapy for LC.
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References
Alter MJ, Mast EE . The epidemiology of viral hepatitis in the United States. Gastroenterol Clin North Am 1994; 23: 437–455.
Hoofnagle JH, di Bisceglie AM . The treatment of chronic viral hepatitis. N Engl J Med 1997; 336: 347–356.
Schalm SW, Fattovich G, Brouwer JT . Therapy of hepatitis C: patients with cirrhosis. Hepatology 1997; 26: 128S–132S.
Bonkovsky HL . Therapy of hepatitis C: other options. Hepatology 1997; 26: 143S–151S.
Di Bisceglie AM et al. Recombinant interferon alfa therapy for chronic hepatitis C. A randomized, double-blind, placebo-controlled trial. N Engl J Med 1989; 321: 1506–1510.
Poynard T et al. Meta-analysis of interferon randomized trials in the treatment of viral hepatitis C: effects of dose and duration. Hepatology 1996; 24: 778–789.
Fort J et al. Effects of long-term administration of interferon alpha in two models of liver fibrosis in rats. J Hepatol 1998; 29: 263–270.
Camps J et al. Randomised trial of lymphoblastoid alpha-interferon in chronic hepatitis C. Effects on inflammation, fibrogenesis and viremia. J Hepatol 1993; 17: 390–396.
Castilla A, Prieto J, Fausto N . Transforming growth factors beta 1 and alpha in chronic liver disease. Effects of interferon alfa therapy. N Engl J Med 1991; 324: 933–940.
Dufour JF, DeLellis R, Kaplan MM . Regression of hepatic fibrosis in hepatitis C with long-term interferon treatment. Dig Dis Sci 1998; 43: 2573–2576.
Okanoue T et al. Interferon therapy lowers the rate of progression to hepatocellular carcinoma in chronic hepatitis C but not significantly in an advanced stage: a retrospective study in 1148 patients. Viral Hepatitis Therapy Study Group. J Hepatol 1999; 30: 653–659.
Schvarcz R et al. Histological outcome in interferon alpha-2b treated patients with chronic posttransfusion non-A, non-B hepatitis. Liver 1991; 11: 30–38.
Yagura M et al. Changes of liver fibrosis in chronic hepatitis C patients with no response to interferon-alpha therapy: including quantitative assessment by a morphometric method. J Gastroenterol 2000; 35: 105–111.
Uemura I . Pharmacokinetics of recombinant human interferon alpha in rat. Kiso to Rinsho 1985; 19: 205–212.
Nakamura T, Akiyoshi H, Saito I, Sato K . Adenovirus-mediated gene expression in the septal cells of cirrhotic rat livers. J Hepatol 1999; 30: 101–106.
Jezequel AM et al. A morphological study of the early stages of hepatic fibrosis induced by low doses of dimethylnitrosamine in the rat. J Hepatol 1987; 5: 174–181.
Friedman SL . Seminars in medicine of the Beth Israel Hospital, Boston. The cellular basis of hepatic fibrosis. Mechanisms and treatment strategies. N Engl J Med 1993; 328: 1828–1835.
Jenkins SA et al. A dimethylnitrosamine-induced model of cirrhosis and portal hypertension in the rat. J Hepatol 1985; 1: 489–499.
Lopez-De Leon A, Rojkind M . A simple micromethod for collagen and total protein determination in formalin-fixed paraffin-embedded sections. J Histochem Cytochem 1985; 33: 737–743.
Bruck R et al. Prevention of hepatic cirrhosis in rats by hydroxyl radical scavengers. J Hepatol 2001; 35: 457–464.
Woessner JF . The determination of hydroxyproline in tissue and protein samples containing small proportion of imino acid. Arch Biochem Biophys 1961; 93: 440–447.
Fujimoto J, Kaneda Y . Reversing liver cirrhosis: impact of gene therapy for liver cirrhosis. Gene Ther 1999; 6: 305–306.
Lee HS et al. Expression of matrix metalloproteinases in spontaneous regression of liver fibrosis. Hepatogastroenterology 2001; 48: 1114–1117.
Watanabe T et al. Gene expression of interstitial collagenase in both progressive and recovery phase of rat liver fibrosis induced by carbon tetrachloride. J Hepatol 2000; 33: 224–235.
Overall CM, Wrana JL, Sodek J . Transcriptional and post-transcriptional regulation of 72-kDa gelatinase/type IV collagenase by transforming growth factor-beta 1 in human fibroblasts. Comparisons with collagenase and tissue inhibitor of matrix metalloproteinase gene expression. J Biol Chem 1991; 266: 14064–14071.
Poncelet AC, Schnaper HW . Regulation of human mesangial cell collagen expression by transforming growth factor-beta 1. Am J Physiol 1998; 275: F458–F466.
Ikeda K et al. In vitro migratory potential of rat quiescent hepatic stellate cells and its augmentation by cell activation. Hepatology 1999; 29: 1760–1767.
Iredale JP et al. Tissue Inhibitor of metalloproteinase-1 messenger RNA expression is enhanced relative to interstitial collagenase messenger RNA in experimental liver injury and fibrosis. Hepatology 1996; 24: 176–184.
Qi Z et al. Blockade of type beta transforming growth factor signaling prevents liver fibrosis and dysfunction in the rat. Proc Natl Acad Sci USA 1999; 96: 2345–2349.
Ueki T et al. Hepatocyte growth factor gene therapy of liver cirrhosis in rats. Nat Med 1999; 5: 226–230.
Rudolph KL et al. Inhibition of experimental liver cirrhosis in mice by telomerase gene delivery. Science 2000; 287: 1253–1258.
Salgado S et al. Liver cirrhosis is reverted by urokinase-type plasminogen activator gene therapy. Mol Ther 2001; 2: 545–551.
Takayama H et al. Diverse tumorigenesis associated with aberrant development in mice overexpressing hepatocyte growth factor/scatter factor. Proc Natl Acad Sci USA 1997; 94: 701–706.
Eto T, Takahashi H . Enhanced inhibition of hepatitis B virus production by asialoglycoprotein receptor-directed interferon. Nat Med 1999; 5: 577–581.
Protzer U et al. Interferon gene transfer by a hepatitis B virus vector efficiently suppresses wild-type virus infection. Proc Natl Acad Sci USA 1999; 96: 10818–10823.
de Roos WK et al. Isolated-organ perfusion for local gene delivery: efficient adenovirus-mediated gene transfer into the liver. Gene Ther 1997; 4: 55–62.
Ferry N, Heard JM . Liver-directed gene transfer vectors. Hum Gene Ther 1998; 9: 1975–1981.
Garcia-Banuelos J et al. Cirrhotic rat livers with extensive fibrosis can be safely transduced with clinical-grade adenoviral vectors. Evidence of cirrhosis reversion. Gene Ther 2002; 9: 127–134.
Nakatani T et al. Assessment of efficiency and safety of adenovirus mediated gene transfer into normal and damaged murine livers. Gut 2000; 47: 563–570.
Akli S et al. Transfer of a foreign gene into the brain using adenovirus vectors. Nat Genet 1993; 3: 224–228.
Hermens WT, Verhaagen J . Adenoviral vector-mediated gene expression in the nervous system of immunocompetent Wistar and T cell-deficient nude rats: preferential survival of transduced astroglial cells in nude rats. Hum Gene Ther 1997; 8: 1049–1063.
Anderson WF . Human gene therapy. Nature 1998; 392: 25–30.
Haecker SE et al. In vivo expression of full-length human dystrophin from adenoviral vectors deleted of all viral genes. Hum Gene Ther 1996; 7: 1907–1914.
Lieber A, He CY, Kirillova I, Kay MA . Recombinant adenoviruses with large deletions generated by Cre-mediated ex-cision exhibit different biological properties compared with first-generation vectors in vitro and in vivo. J Virol 1996; 70: 8944–8960.
Langer R . Drug delivery and targeting. Nature 1998; 392: 5–10.
Miyake S et al. Efficient generation of recombinant adenoviruses using adenovirus DNA–terminal protein complex and a cosmid bearing the full-length virus genome. Proc Natl Acad Sci USA 1996; 93: 1320–1324.
Aoki K et al. Restricted expression of an adenoviral vector encoding Fas ligand (CD95L) enhances safety for cancer gene therapy. Mol Ther 2000; 1: 555–565.
Niwa H, Yamamura K, Miyazaki J . Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 1991; 108: 193–199.
Aoki K et al. Polyethylenimine-mediated gene transfer into pancreatic tumor dissemination in the murine peritoneal cavity. Gene Ther 2001; 8: 508–514.
Acknowledgements
This work was supported in part by a grant-in-aid for the 2nd Term Comprehensive 10-year Strategy for Japan, and by grants-in-aid for Cancer Research from the Ministry of Health, Labour and Welfare of Japan.
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Suzuki, K., Aoki, K., Ohnami, S. et al. Adenovirus-mediated gene transfer of interferon α improves dimethylnitrosamine-induced liver cirrhosis in rat model. Gene Ther 10, 765–773 (2003). https://doi.org/10.1038/sj.gt.3301949
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DOI: https://doi.org/10.1038/sj.gt.3301949
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