|
 |
|
|
|
EMBO reports 5, 7, 728–733 (2004)
doi:10.1038/sj.embor.7400171
Published online: 11 June 2004
Efficient production of germline transgenic chickens using lentiviral vectors
Michael J McGrew1, Adrian Sherman1, Fiona M Ellard2, Simon G Lillico1, Hazel J Gilhooley1, Alan J Kingsman2, Kyriacos A Mitrophanous2 & Helen Sang1
|
|
|
|
1 Roslin Institute, Roslin, Midlothian EH25 9PS, UK
2 Oxford Biomedica (UK) Ltd, Medawar Centre, The Oxford Science Park, Oxford OX4 4GA, UK
To whom correspondence should be addressed
Helen Sang Tel: +44 131 527 4234; Fax: +44 131 440 0434; E-mail: helen.sang@bbsrc.ac.uk
Received 27 January 2004; Accepted 26 April 2004; Published online 11 June 2004.
|
|
|
|
Abstract
An effective method for genetic modification of chickens has yet to be developed. An efficient technology, enabling production of transgenic birds at high frequency and with reliable expression of transgenes, will have many applications, both in basic research and in biotechnology. We investigated the efficiency with which lentiviral vectors could transduce the chicken germ line and examined the expression of introduced reporter transgenes. Ten founder cockerels transmitted the vector to between 4% and 45% of their offspring and stable transmission to the G2 generation was demonstrated. Analysis of expression of reporter gene constructs in several transgenic lines showed a conserved expression profile between individuals that was maintained after transmission through the germ line. These data demonstrate that lentiviral vectors can be used to generate transgenic lines with an efficiency in the order of 100-fold higher than any previously published method, with no detectable silencing of transgene expression between generations.
|
 |
|
Top of page MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated | |
|
top  |
|
|
|
