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| Subject Categories: Signal Transduction | Development |
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| The EMBO Journal
(2007) 26, 2513–2526, doi:10.1038/sj.emboj.7601688 Published online 3 May 2007 |
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Essential role for  -arrestin 2 in the regulation of Xenopus convergent extension movements |
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| Gun-Hwa Kim and Jin-Kwan Han |
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Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Kyungbuk, Republic of Korea To whom correspondence should be addressed Jin-Kwan Han, Department of Life Science, Pohang University of Science and Technology, San 31, Hyoja Dong, Pohang, Kyungbuk 790-784, Republic of Korea. Tel.: +82 54 279 2126; Fax: +82 54 279 2199; E-mail: jkh@postech.ac.kr Received 25 October 2006; Accepted 26 March 2007; Published online 3 May 2007. |
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| Abstract |
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| -Arrestin 2 (arr2) is a multifunctional protein that regulates numerous aspects of G-protein-coupled receptor function. However, its possible involvement in developmental processes is poorly understood. In this work, we examined the potential role of arr2 during Xenopus early development. Gain- and loss-of-function studies showed that Xenopus arr2 (xarr2) is required for proper convergent extension (CE) movements, and normal cell polarization and intercalation without affecting cell fate. Moreover, for CE movements, arr2 acts as an essential regulator of dishevelled-mediated PCP (planar cell polarity) signaling, but not G-protein-mediated Ca2+ signaling. Notably, xarr2 is localized with the same distribution as the dishevelled protein, which is reasonable, as xarr2 is required for dishevelled activation of RhoA. Furthermore, xarr2 interacts with the N-terminal quarter of Daam1 and RhoA proteins, but not Rac1, and regulates RhoA activation through Daam1 activation for CE movements. We provide evidence that the endocytic activity of xarr2 is essential for control of CE movements. Taken together, our results suggest that arr2 has a pivotal role in the regulation of Xenopus CE movements. |
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| Keywords: -arrestin 2, convergent extension movements, planar cell polarity, Wnt pathway,
Xenopus development |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated NEWS AND VIEWSNature Cell Biology News and Views (01 Nov 2002) A no-Wnt situation: SFRPs as axon guidance molecules Nature Neuroscience News and Views (01 Oct 2005) RESEARCHEssential role for β-arrestin 2 in the regulation of Xenopus convergent extension movements The EMBO Journal Article WGEF activates Rho in the Wnt?PCP pathway and controls convergent extension in Xenopus gastrulation The EMBO Journal Article |
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