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| Subject Categories: Structural Biology | Genome Stability & Dynamics |
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| The EMBO Journal
(2005) 24, 906–918, doi:10.1038/sj.emboj.7600582 Published online 17 February 2005 |
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| Crystal structure and DNA-binding analysis of RecO from Deinococcus radiodurans |
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| Ingar Leiros1, Joanna Timmins1, David R Hall and Sean McSweeney |
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Macromolecular Crystallography Group, European Synchrotron Radiation Facility, Grenoble, France To whom correspondence should be addressed Sean McSweeney, Macromolecular Crystallography Group, European Synchrotron Radiation Facility, BP 220, 6 rue Jules Horowitz, 38043 Grenoble Cedex 9, France. Tel.: +33 4 76 88 23 62; Fax: +33 4 76 88 21 60; E-mail: mcsweeney@esrf.fr 1 These authors contributed equally to this work Received 16 August 2004; Accepted 21 January 2005; Published online 17 February 2005. |
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| Abstract |
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| The RecFOR pathway has been shown to be essential for DNA repair through the process of homologous recombination in bacteria and, recently, to be important in the recovery of stalled replication forks following UV irradiation. RecO, along with RecR, RecF, RecQ and RecJ, is a principal actor in this fundamental DNA repair pathway. Here we present the three-dimensional structure of a member of the RecO family. The crystal structure of Deinococcus radiodurans RecO (drRecO) reveals possible binding sites for DNA and for the RecO-binding proteins within its three discrete structural regions: an N-terminal oligonucleotide/oligosaccharide-binding domain, a helical bundle and a zinc-finger motif. Furthermore, drRecO was found to form a stable complex with RecR and to bind both single- and double-stranded DNA. Mutational analysis confirmed the existence of multiple DNA-binding sites within the protein. |
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| Keywords: Cys4 zinc-finger, DNA repair, OB fold, RecFOR pathway, RecO |
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Top of page MORE ARTICLES LIKE THISThese links to content published by NPG are automatically generated NEWS AND VIEWSCommon fold, common function, common origin? Nature Structural Biology News and Views (01 Mar 1997) RESEARCHCrystal structure and mutational study of RecOR provide insight into its mode of DNA binding The EMBO Journal Article (11 Jul 2007) Crystal structure and mutational study of RecOR provide insight into its mode of DNA binding The EMBO Journal Article Ring-shaped architecture of RecR: implications for its role in homologous recombinational DNA repair The EMBO Journal Article (19 May 2004) |
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