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Selective killing of cancer cells based on translational control of a suicide gene

Cancer Gene Therapy volume 9, pages 573–578 (2002)Cite this article

Abstract

The translation initiation factor, eIF4E, is commonly overexpressed in solid tumors. This elevation allows for efficient translation of mRNA that are normally repressed by their 5′ untranslated region, many of which encode growth-promoting proteins. This property was exploited to modulate the synthesis of HTK at the translational level to selectively kill cancer cells. Various breast cancer cell lines can efficiently synthesize HTK from the translationally regulated mRNA, whereas normal cells cannot. Accordingly, only cancer cells were killed at low concentrations of ganciclovir. By altering the expression of eIF4E, it was possible to modulate the sensitivity of various cell lines to ganciclovir.

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Acknowledgements

This work was supported by NIH Grant CA69148.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology and the Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, Shreveport, 71130-3932, Louisiana, USA

    Robert J DeFatta, Yuan Li & Arrigo De Benedetti

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  1. Robert J DeFatta
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  2. Yuan Li
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  3. Arrigo De Benedetti
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Correspondence to Arrigo De Benedetti.

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DeFatta, R., Li, Y. & De Benedetti, A. Selective killing of cancer cells based on translational control of a suicide gene. Cancer Gene Ther 9, 573–578 (2002). https://doi.org/10.1038/sj.cgt.7700468

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