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Successful cryopreservation of purified autologous CD34+ cells: influence of freezing parameters on cell recovery and engraftment

Abstract

Conventional hematopoietic stem cell cryopreservation methods use a DMSO concentration of 10%. However, cells manipulated ex vivo may require more refined freezing protocols adapted to the specific cell suspension. In this retrospective study, we evaluated the results obtained with CD34+ cells purified from peripheral blood of 39 patients on the CEPRATE SC System and frozen in 7.5% DMSO with a view to transplantation. The post-freezing recovery of progenitor cells was 89.4 ± 27.87% for CD34+ cells, 59.13 ± 36.93% for CFU-GM, and 53.49 ± 40.71 for BFU-E. Neither the purity of the suspension nor the nucleated cell density during freezing was predictive of cell recovery. No difference was observed between cells stored in vials and bags. Thirty-seven patients transplanted with the concentrated CD34+ fraction received 4.46 × 106 CD34+ cells/kg and 33.04 × 104 CFU-GM/kg. The median time to granulocyte (>0.5 × 109/l) and platelet (>50 × 109/l) engraftment was 11 and 13 days, respectively. Only cell density and the infused number of CD34+ cells and CFU-GM were significantly related to hematological recovery. Our data suggest that purified CD34+ cells can be successfully cryopreserved in 7.5% DMSO and may represent a first step in establishing freezing parameters for selected CD34+ cells.

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Beaujean, F., Bourhis, JH., Bayle, C. et al. Successful cryopreservation of purified autologous CD34+ cells: influence of freezing parameters on cell recovery and engraftment. Bone Marrow Transplant 22, 1091–1096 (1998). https://doi.org/10.1038/sj.bmt.1701494

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  • DOI: https://doi.org/10.1038/sj.bmt.1701494

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