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AGC kinases regulate phosphorylation and activation of eukaryotic translation initiation factor 4B

Oncogene volume 28, pages 95–106 (2009)Cite this article

Abstract

Eukaryotic translation initiation factor 4B (eIF4B) plays a critical role during the initiation of protein synthesis and its activity can be regulated by multiple phosphorylation events. In a search for novel protein kinase B (PKB/c-akt) substrates, we identified eIF4B as a potential target. Using an in vitro kinase assay, we found that PKB can directly phosphorylate eIF4B on serine 422 (ser422). Activation of a conditional PKB mutant, interleukin-3 (IL-3) or insulin stimulation resulted in PKB-dependent phosphorylation of this residue in vivo. This was prevented by pretreatment of cells with the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 or pharmacological inhibition of PKB. Pretreatment of cells with rapamycin, inhibiting mTOR or U0126 to inhibit MEK, had little effect on eIF4B ser422 phosphorylation. In contrast, following amino-acid refeeding, eIF4B ser422 phosphorylation was found to be mammalian target of rapamycin (mTOR)-dependent. We further identified eIF4B ser406 as a novel mitogen-regulated phosphorylation site. Insulin-induced phosphorylation of eIF4B ser406 was dependent on both MEK and mTOR activity. Utilizing a novel translational control luciferase assay, we could further demonstrate that phosphorylation of ser406 or ser422 is essential for optimal translational activity of eIF4B. These data provide novel insights into complex multikinase regulation of eIF4B phosphorylation and reveal an important mechanism by which PKB can regulate translation, potentially critical for the transforming capacity of this AGC kinase family member.

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Acknowledgements

AGM van Gorp and KE van der Vos were supported by a grant from the Dutch Scientific Organization (NWO; ZonMw 917.36.316).

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Author notes

  1. A G M van Gorp

    Present address: 7Current address: Netherlands Forensic Institute, the Hague, The Netherlands.,

  2. A G M van Gorp and K E van der Vos: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Immunology, Molecular Immunology Laboratory, University Medical Center Utrecht, Utrecht, The Netherlands

    A G M van Gorp, K E van der Vos & P J Coffer

  2. Department of Endocrinology and Metabolic Diseases, University Medical Center Utrecht, Utrecht, The Netherlands

    A B Brenkman & N van den Broek

  3. Leibniz Institute for Age Research–Fritz Lipmann Institute, Beutenbergstr, Jena, Germany

    A Bremer & C F Calkhoven

  4. Department of Physiological Chemistry, Centre for Biomedical Genetics, University Medical Center Utrecht, Utrecht, The Netherlands

    F Zwartkruis & B M T Burgering

  5. Department of Biological Chemistry, School of Medicine, University of California, Davis, CA, USA

    J W Hershey

  6. Department of Pediatric Immunology, University Medical Center Utrecht, Utrecht, The Netherlands

    P J Coffer

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  1. A G M van Gorp
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Correspondence to P J Coffer.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc)

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van Gorp, A., van der Vos, K., Brenkman, A. et al. AGC kinases regulate phosphorylation and activation of eukaryotic translation initiation factor 4B. Oncogene 28, 95–106 (2009). https://doi.org/10.1038/onc.2008.367

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