Abstract
In this report, we examined the effect of increased target site access on activated human RNA-induced silencing complex (RISC*) catalysis. Kinetic studies revealed that siRNA-programmed RISC* cleaved target RNA with higher efficiencies when target site access was increased. These results provide evidence that target site access is linked to RISC* catalysis.
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Acknowledgements
We thank members of the Rana lab, C. Mello and P. Zamore for helpful discussions; T.J. Richman for editorial assistance; and H. Cao for kinetic data analysis. This work was supported in part by grants from the US National Institutes of Health (AI 41404 and AI 43198) to T.M.R.
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Supplementary information
Supplementary Fig. 1
Cdk9 RISC concentration. (PDF 295 kb)
Supplementary Fig. 2
GFP RISC kinetics. (PDF 209 kb)
Supplementary Fig. 3
GFP and Cdk9 initial velocity. (PDF 495 kb)
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Brown, K., Chu, Cy. & Rana, T. Target accessibility dictates the potency of human RISC. Nat Struct Mol Biol 12, 469–470 (2005). https://doi.org/10.1038/nsmb931
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DOI: https://doi.org/10.1038/nsmb931
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