Nature Methods
- 4, 39 - 42 (2007)
Published online: 26 November 2006; | doi:10.1038/nmeth975
Fast manipulation of cellular cAMP level by light in vivoSaskia Schröder-Lang1, Martin Schwärzel2, Reinhard Seifert3, Timo Strünker3, Suneel Kateriya4, Jens Looser5, Masakatsu Watanabe6, 7, U Benjamin Kaupp3, Peter Hegemann4 & Georg Nagel1, 51
Max-Planck-Institut für Biophysik, 60439 Frankfurt, Germany. 2
Saarland Universität, Naturwissenschaftlich-Technische Fakultät III, FR 8.3, Zoologie & Physiologie, 66041 Saarbrücken, Germany. 3
Forschungszentrum Jülich, Institut für Neurowissenschaften und Biophysik, Abteilung Zelluläre Signalverarbeitung, INB-1, 52425 Jülich, Germany. 4
Humboldt Universität zu Berlin, Experimentelle Biophysik, 10115 Berlin, Germany. 5
Universität Würzburg, Julius-von-Sachs-Institut für Biowissenschaften, 97082 Würzburg, Germany. 6
National Institute for Basic Biology, Okazaki, 444-8585, Japan. 7
School of Advanced Sciences, Graduate University of Advanced Studies (SOKENDAI), Hayama, 240-0193, Japan.
Correspondence should be addressed to Georg Nagel nagel@botanik.uni-wuerzburg.de or Martin Schwärzel m.schwaerzel@mx.uni-saarland.de The flagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the flavoproteins PAC and PAC . Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells.
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