Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Commentary
  • Published:

Next-generation sequencing transforms today's biology

A new generation of non-Sanger-based sequencing technologies has delivered on its promise of sequencing DNA at unprecedented speed, thereby enabling impressive scientific achievements and novel biological applications. However, before stepping into the limelight, next-generation sequencing had to overcome the inertia of a field that relied on Sanger-sequencing for 30 years.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

References

  1. Sanger, F. et al. Nature 24, 687–695 (1977).

    Article  Google Scholar 

  2. Sanger, F., Nicklen, S. & Coulson, A.R. Proc. Natl. Acad. Sci. USA 74, 5463–5467 (1977).

    Article  CAS  Google Scholar 

  3. Maxam, A.M. & Gilbert, W. Proc. Natl. Acad. Sci. USA 74, 560–564 (1977).

    Article  CAS  Google Scholar 

  4. Sanger, F. & Coulson, A.R. J. Mol. Biol. 94, 441–448 (1975).

    Article  CAS  Google Scholar 

  5. Margulies, M. et al. Nature 437, 376–380 (2005).

    Article  CAS  Google Scholar 

  6. Shendure, J. et al. Science 309, 1728–1732 (2005).

    Article  CAS  Google Scholar 

  7. Edwards, R.A. et al. BMC Genomics 7, 57 (2006).

    Article  Google Scholar 

  8. Sogin, M.L. et al. Proc. Natl. Acad. Sci. USA 103, 12115–12120 (2006).

    Article  CAS  Google Scholar 

  9. Angly, F.E. et al. PLoS Biol. 4, 2121–2131 (2006).

    Article  CAS  Google Scholar 

  10. Poinar, H.N. et al. Science 311, 392–394 (2006).

    Article  CAS  Google Scholar 

  11. Green, R.E. et al. Nature 444, 330–336 (2006).

    Article  CAS  Google Scholar 

  12. Noonan, J.P. et al. Science 314, 1113–1118 (2006).

    Article  CAS  Google Scholar 

  13. Andries, K. et al. Science 307, 223–227 (2005).

    Article  CAS  Google Scholar 

  14. Velicer, G.J. et al. Proc. Natl. Acad. Sci. USA 103, 8107–8112 (2006).

    Article  CAS  Google Scholar 

  15. Goldberg, S.M. et al. Proc. Natl. Acad. Sci. USA 103, 11240–11245 (2006).

    Article  CAS  Google Scholar 

  16. Gilbert, M.T.P. et al. Science 317, 1927–1930 (2007).

    CAS  Google Scholar 

  17. Cheung, F. et al. BMC Genomics 7, 272 (2006).

    Article  Google Scholar 

  18. Ohtsu, K. et al. Plant J. 52, 391–404 (2007).

    Article  CAS  Google Scholar 

  19. Thomas, R.K. et al. Nat. Genet. 39, 347–351 (2007).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

I thank W. Miller and N. Wittekindt for suggestions on the manuscript. The author is funded in part by the Gordon and Betty Moore Foundation.

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schuster, S. Next-generation sequencing transforms today's biology. Nat Methods 5, 16–18 (2008). https://doi.org/10.1038/nmeth1156

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth1156

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing