Nature Methods
- 5, 405 - 407 (2008)
Published online: 20 April 2008; | doi:10.1038/nmeth.1204
Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidaseNadia Taouatas, Madalina M Drugan, Albert J R Heck & Shabaz Mohammed
Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands.
Correspondence should be addressed to Albert J R Heck a.j.r.heck@uu.nl or Shabaz Mohammed s.mohammed@uu.nl We introduce a method for sequencing peptides by mass spectrometry using a metalloendopeptidase that cleaves proteins at the amino side of lysine (Lys-N). When analyzed by electron transfer dissociation (ETD)–based mass spectrometric sequencing, Lys-N–digested peptides that contain a single lysine residue produce spectra dominated by c-type fragment ions, providing simple ladders for sequence determination. This method should be a valuable strategy for de novo sequencing and the analysis of post-translational modifications.
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