Abstract
Type 1 helper T (TH1) cells are essential for cellular immunity, but their ontogeny, maturation and durability remain poorly understood. By constructing a dominant-negative form of T-bet, we were able to determine the role played by this lineage-inducing trans-activator in the establishment and maintenance of heritable TH1 gene expression. Optimal induction of interferon-γ (IFN-γ) expression required genetic interaction between T-bet and its target, the homeoprotein Hlx. In fully mature TH1 cells, reiteration of IFN-γ expression and stable chromatin remodeling became relatively independent of T-bet activity and coincided with demethylation of DNA. In contrast, some lineage attributes, such as expression of IL-12Rβ2 (interleukin 12 receptor β2), required ongoing T-bet activity in mature TH1 cells and their progeny. These findings suggest that heritable states of gene expression might be maintained by continued expression of the inducing factor or by a mechanism that confers a stable imprint of the induced state.
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Acknowledgements
We thank M. Weiss for helpful discussion; W. Pear and B. Cobb for advice on retrovirus production; V. Amorosa, J. Hartman, E. L. Pearce, M. Kessler and A. Villarino for assistance; D. Kessler for the cDNA of Drosophila engrailed repression domain; and W. DeMuth for cell sorting. Supported by the NIH (AI42370 to SLR).
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Mullen, A., Hutchins, A., High, F. et al. Hlx is induced by and genetically interacts with T-bet to promote heritable TH1 gene induction. Nat Immunol 3, 652–658 (2002). https://doi.org/10.1038/ni807
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DOI: https://doi.org/10.1038/ni807
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