Nature Genetics
36, 304 - 312 (2004)
Published online: 15 February 2004; | doi:10.1038/ng1306
Identification and validation of PDGF transcriptional targets by microarray-coupled gene-trap mutagenesisWeisheng V Chen1, 2, Jeffrey Delrow3, Philip D Corrin1, Jason P Frazier1
& Philippe Soriano11
Program in Developmental Biology, Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA. 2
Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA. 3
Genomics Resource, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
Correspondence should be addressed to Philippe Soriano psoriano@fhcrc.orgWe developed a versatile, high-throughput genetic screening strategy by coupling gene mutagenesis and expression profiling technologies. Using a retroviral gene-trap vector optimized for efficient mutagenesis and cloning, we randomly disrupted genes in mouse embryonic stem (ES) cells and amplified them to construct a cDNA microarray. With this gene-trap array, we show that transcriptional target genes of platelet-derived growth factor (PDGF) can be efficiently and reliably identified in physiologically relevant cells and are immediately accessible to genetic studies to determine their in vivo roles and relative contributions to PDGF-regulated developmental processes. The same platform can be used to search for genes of specific biological relevance in a broad array of experimental settings, providing a fast track from gene identification to functional validation.
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