Sequencing project reveals two different versions make a female, one a male.
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References
Beye, M. et al. The gene csd is the primary signal for sexual development in the honey bee and encodes a new SR-type protein. Cell, 114, 419 - 429, (2003).
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US Department of Agriculture Bee Lab
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Whitfield, J. Honeybee sex gene discovered. Nature (2003). https://doi.org/10.1038/news030818-15
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DOI: https://doi.org/10.1038/news030818-15