Polyubiquitination of substrates requires the coordinated and sequential action of three enzymes: E1, E2 and E3. Now, a study shows that activated E2 associates transiently with E3, and that subsequent release of the E2 is functionally required for substrate polyubiquitination.
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Pintard, L., Peter, M. Cdc34: cycling on and off the SCF. Nat Cell Biol 5, 856–857 (2003). https://doi.org/10.1038/ncb1003-856
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DOI: https://doi.org/10.1038/ncb1003-856
