Abstract
Transformants of maize inbred A188 were efficiently produced from immature embryos cocultivated with Agrobacterium tumefaciens that carried “super-binary” vectors. Frequencies of transformation (independent transgenic plants/embryos) were between 5% and 30%. Almost all transformants were normal in morphology, and more than 70% were fertile. Stable integration, expression, and inheritance of the transgenes were confirmed by molecular and genetic analysis. Between one and three copies of the transgenes were integrated with little rearrangement, and the boundaries of T-DNA were similar to those in transgenic dicotyledons and rice. F1 hybrids between A188 and five other inbreds were transformed at low frequencies.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
De Cleene, M. and Deley, J. 1976. The host range of crown gall. Bot. Rev. 42: 389–466.
Grimsley, N., Hohn, T., Davies, J.W. and Hohn, B. 1987. Agrobacterium-mediated delivery of infectious maize streak virus into maize plants. Nature 325: 177–179.
Gould, J., Devey, M., Hasegawa, O., Ulian, E.C., Peterson, G. and Smith, R.H. 1991. Transformation of Zea mays L using Agrobacterium tufemaciens and the shoot apex. Plant Physiol. 95: 426–434.
Shen, W.-H., Escudero, J., Schläppi, M., Ramos, C., Hohn, B. and KoukolikoväNicola, Z. 1993. T-DNA transfer to maize cells: Histochemical investigation of β-glucuronidase activity in maize tissues. Proc. Natl. Acad. Sci. USA 90: 1488–1492.
Chan, M.-T., Lee, T.-M. and Chang, H.-H. 1992. Transformation of indica rice (Oryza sativa L.) mediated by Agrobacterium tumefaciens. Plant Cell Physiol. 33: 577–583.
Chan, M.-T., Chang, H.-H., Ho, S.-L., Tong, W.-F. and Yu, S.-M. 1993. Agrobacterium-mediated production of transgenic rice plants expessing a chimeric α-amylase promoter/β-glucuronidase gene. Plant Mol. Biol. 22: 491–506.
Mooney, P.A., Goodwin, P.B., Dennis, E.S. and Llewellyn, D.J. 1991. Agrobacterium tumafiens-gene transfer into wheat tissues. Plant Cell Tissue Organ Cult. 25: 209–218.
Hiei, Y., Ohta, S., Komari, T. and Kumasho, T. 1994. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal 6: 271–282.
Armstrong, C.L. and Green, C.E. 1985. Established and maintenance of friable, embrygenic maize callus and the involvement of L-proline. Planta 164: 207–214.
Koziel, M.G. et al. 1993. Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thurigiensis . Bio/Technology 11: 194–200.
Wan, Y., Widholdm, J.M. and Lemaux, P.G. 1995. Type I callus as a bombardment target for generating fertile transgenic maize (Zea mays L.) Planta 196: 7–14.
Frame, B.R., Drayton, P.R., Bagnall, S.V., Lewnau, C.J., Bullock, W.P., Wilson, H.M., Dunwell, J.M., Thompson, J.A. and Wang, K. 1994. Production of fertile transgenic maize plants by silicon carbide whisker-mediated transformation. The Plant Journal 6: 941–948.
D'Halluin, K., Bonne, E., Bossut, M., Beuckeleer, M.D. and Leemans, J. 1992. Transgenic maize plants by tissue electroporation. The Plant Cell 4: 1495–1505.
Chu, C.-C. 1978. The N6 medium and its applications to anther culture of cereal crops, pp. 43–50 in Proc. Symp. Plant Tissue Culture. Science Press, Peking.
Linsmaier, E. and Skoog, F. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18: 100–127.
Does, M.R., Dekker, B.M.M., de Groot, M.J.A., and Offringa, R. 1991. A quick method to estimate the T-DNA copy number i transgenic plants at an early stage after transformation, using inverse PCR. Plant Mol. Biol. 17: 151–153.
Yadav, N.S., Vanderleyden, J., Bennett, D.R., Barnes, W.M. and Chilton, M.D. 1982. Short direct repeats flank the T-DNA on a nopaline Ti plasmid. Proc. Natl. Acad. Sci. USA 79: 6322–6326.
Zambryski, P., Depicker, A., Kruger, K. and Goodman, H.M. 1982. Tumor induction by Agrobacterium tumefaciens: analysis of the boundaries of T-DNA. J. Mol. Appl. Genet. 1: 361–370.
Ohta, S., Mita, S., Hattori, T. and Nakamura, K. 1990. Construction and expression in tobacco of a β-glucuronidase (GUS) reporter gene containing an intron within the coding sequence. Plant Cell Physiol. 31: 805–813.
Rhodes, C.A., Pierce, D.A., Mettler, I.J., Mascarenhas, D. and Detmer, J.J. 1988. Genetically transformed maize plants from protoplasts. Science 240: 204–207.
Gordon-Kamm, W.J. et al. 1990. Transformation of maize cells and regeneration of fertile transgenic plants. The Plant Cell 2: 603–618.
Fromm, M.E., Morrish, F., Armstrong, C., Williams, R., Thomas, J. and Klein, T.M. 1990. Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio/Technology 8: 833–839.
Walters, D.A., Vetsch, C.S., Potts, D.E. and Lundquist, R.C. 1992. Transformation and inheritance of a hygromycin phosphotansferase gene in maize plants. Plant Mol. Biol. 18: 189–200.
Komari, T. 1990. Transformation of cultured cells of Chenopodium quinoa by binary vectors that carry a fragment of DNA from the virulence region of pTiBo542. Plant Cell Rep. 9: 303–306.
Denecke, J., Goselé, J., Botterman, J. and Cornelissen, M. 1989. Quantitative analysis of transiently expressed genes in plant cells. Methods in Molecular and Cellular Biology 1: 19–27.
Ditta, G., Stanfield, S., Corbin, D. and Helinski, D.R. 1980. Broad host range DNA cloning system for Gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti . Proc. Natl. Acad. Sci. USA 77: 7347–7351.
Komari, T., Saito, Y., Nakakido, F. and Kumashiro, T. 1989. Efficient selection of somatic hybrids in Nicotiana tabacum L. using a combination of drug-resistance markers introduced by transformation. Theor. Appl. Genet. 77: 547–552.
Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Jefferson, R.A. 1987. Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol. Biol. Rep. 5: 387–405.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ishida, Y., Saito, H., Ohta, S. et al. High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens. Nat Biotechnol 14, 745–750 (1996). https://doi.org/10.1038/nbt0696-745
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/nbt0696-745
This article is cited by
-
Agrobacterium tumefaciens-Mediated Plant Transformation: A Review
Molecular Biotechnology (2023)
-
Enigma of recalcitrance to tissue culture in the oilseed crop Sesamum indicum L.—a review
Plant Cell, Tissue and Organ Culture (PCTOC) (2023)
-
Advances in bread wheat production through CRISPR/Cas9 technology: a comprehensive review of quality and other aspects
Planta (2023)
-
LaCl3 treatment improves Agrobacterium-mediated immature embryo genetic transformation frequency of maize
Plant Cell Reports (2022)
-
Agrobacterium-mediated In-planta transformation of bread wheat (Triticum aestivum L.)
Journal of Plant Biochemistry and Biotechnology (2022)