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Enhanced discrimination of single nucleotide polymorphisms by artificial mismatch hybridization

Nature Biotechnology volume 15, pages 331–335 (1997)Cite this article

Abstract

In order to increase the discrimination of single nucleotide polymorphisms in DNA hybridization, artificial mismatches are inserted into probe oligonucleotides using the base analog 3-nitropyrrole. Differences in thermal stability (ΔTm) between hybrids formed with normal and single-nucleotide-variant DNA targets are increased by as much as 200% over conventional hybridization, and are strongly dependent upon the spacing between mismatches. The increased specificity is demonstrated by hybridization analysis and allele-specific amplification within the HLA-DRB locus.

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Author notes

  1. Lloyd M. Smith: Corresponding author (e-mail: smith@chem.wisc.edu).

Authors and Affiliations

  1. Department of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706

    Zhen Guo, Qinghua Liu & Lloyd M. Smith

Authors
  1. Zhen Guo
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  2. Qinghua Liu
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  3. Lloyd M. Smith
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Guo, Z., Liu, Q. & Smith, L. Enhanced discrimination of single nucleotide polymorphisms by artificial mismatch hybridization. Nat Biotechnol 15, 331–335 (1997). https://doi.org/10.1038/nbt0497-331

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  • DOI: https://doi.org/10.1038/nbt0497-331

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