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Prohepcidin concentrations and erythroid progenitors in cord blood of appropriate versus small for gestational age neonates

Abstract

Objective:

Prohepcidin (Pro-Hep), synthesized in the liver, is the prohormone of hepcidin (Hep), which reduces iron absorption in the gut; its synthesis is enhanced by inflammation and is reduced during hypoxia. We aimed to study the hypothesis that infants born small for gestational age (SGA) have reduced cord blood concentrations of Pro-Hep.

Study Design:

Cord blood was collected from 20 SGA (term and near term >35 week gestation) infants and 20 appropriate for gestational age (AGA) controls. We excluded infants exposed to maternal chronic diseases, smoking, diabetes, alcohol or drug use. Both groups had a 1 min Apgar score above or equal to 7 and had normal cord blood pH (above 7.25). ELISA was used to determine serum concentrations of Pro-Hep and erythropoietin (EPO). Circulating CD71+/CD45/SSClow cells were measured by flow cytometry as an index of erythroid progenitors.

Result:

There were no significant differences between groups in terms of hemoglobin concentrations, and Pro-Hep. In contrast, EPO levels and circulating CD71+/CD45/SSClow erythroid progenitors were significantly higher in the SGA group. These differences remained significant even after controlling for gestational age and gravidity.

Conclusion:

Contrary to EPO upregulation during intrauterine growth restriction (IUGR), and higher concentrations of circulating erythroid progenitors, Pro-Hep concentration is not affected by IUGR.

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References

  1. Ganz T . Hepcidin and its role in regulating systemic iron metabolism. Hematology Am Soc Hematol Educ Program 2008; 507: 29–35.

    Google Scholar 

  2. Nicolas G, Chauvet C, Viatte L, Danan JL, Bigard X, Devaux I et al. The gene encoding the iron regulatory peptide hepcidin is regulated by anemia, hypoxia, and inflammation. J Clin Invest 2002; 110: 1037–1044.

    Article  CAS  Google Scholar 

  3. Eleftheriadis T, Liakopoulos V, Antoniadi G, Kartsios C, Stefanidis I . The role of hepcidin in iron homeostasis and anemia in hemodialysis patients. Semin Dial 2009; 22: 70–77.

    Article  Google Scholar 

  4. Tiker F, Celik B, Tarcan A, Kilicdag H, Ozbek N, Gurakan B . Serum pro-hepcidin levels and relationships with iron parameters in healthy preterm and term newborns. Pediatr Hematol Oncol 2006; 23: 293–297.

    Article  CAS  Google Scholar 

  5. Balogh A, Szabó M, Kelen D, Bokodi G, Prechl J, Bösze S et al. Prohepcidin levels during human perinatal adaptation. Pediatr Hematol Oncol 2007; 24: 361–368.

    Article  CAS  Google Scholar 

  6. Hecher K, Snijders R, Campbell S, Nicolaides K . Fetal venous, intracardiac, and arterial blood flow measurements in intrauterine growth retardation: relationship with fetal blood gases. Am J Obstet Gynecol 1995; 173: 10–15.

    Article  CAS  Google Scholar 

  7. Jazayeri A, Tsibris JC, Spellacy WN . Fetal erythropoietin levels in growth-restricted and appropriately grown neonates with and without abnormal fetal heart rate tracings: a comparison with cord blood gases and Apgar scores. J Perinatol 1999; 19: 255–259.

    Article  CAS  Google Scholar 

  8. Shuper A, Mimouni F, Merlob P, Zaizov R, Reisner SH . Thrombocytopenia in small-for-gestational-age infants. Acta Paediatr Scand 1983; 72: 139–140.

    Article  CAS  Google Scholar 

  9. Meberg A, Orstavik I, Sövde A . Erythroblastemia and thrombocytopenia in small for gestational age infants: relation to intrauterine hypoxia, infections and maternal smoking. Acta Paediatr Belg 1978; 31: 213–218.

    CAS  PubMed  Google Scholar 

  10. Dollberg S, Haklai Z, Mimouni FB, Gorfein I, Gordon ES . Birth weight standards in the live-born population in Israel. Isr Med Assoc J 2005; 7: 311–314.

    PubMed  Google Scholar 

  11. Sivertsen EA, Hystad ME, Gutzkow KB, Døsen G, Smeland EB, Blomhoff HK et al. PI3K/Akt-dependent Epo-induced signalling and target genes in human early erythroid progenitor cells. Br J Haematol 2006; 135: 117–128.

    Article  CAS  Google Scholar 

  12. Axt-Fliedner R, Hendrik HJ, Schmidt W . Nucleated red blood cell counts in growth-restricted neonates with absent or reversed-end-diastolic umbilical artery velocity. Clin Exp Obstet Gynecol 2002; 29: 242–246.

    CAS  PubMed  Google Scholar 

  13. Kiserud T . Physiology of the fetal circulation. Semin Fetal Neonatal Med 2005; 10: 493–503.

    Article  Google Scholar 

  14. Kulaksiz H, Theilig F, Bachmann S, Gehrke SG, Rost D, Janetzko A et al. The iron-regulatory peptide hormone hepcidin: expression and cellular localization in the mammalian kidney. J Endocrinol 2005; 184: 361–370.

    Article  CAS  Google Scholar 

  15. Valore EV, Ganz T . Posttranslational processing of hepcidin in human hepatocytes is mediated by the prohormone convertase furin. Blood Cells Mol Dis 2008; 40: 132–138.

    Article  CAS  Google Scholar 

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Correspondence to D Mandel.

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Amarilyo, G., Mimouni, F., Oren, A. et al. Prohepcidin concentrations and erythroid progenitors in cord blood of appropriate versus small for gestational age neonates. J Perinatol 30, 396–398 (2010). https://doi.org/10.1038/jp.2009.179

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  • DOI: https://doi.org/10.1038/jp.2009.179

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